Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-Phenyl-2-thiourea | 1 mM, 94% inhibition | Aspergillus nidulans | |
8-hydroxyquinoline | 3 mM, 44% inhibition | Aspergillus nidulans | |
azide | no inhibition by sodium azide | Aspergillus nidulans | |
diethyldithiocarbamate | 0.1 mM, complete inhibition | Aspergillus nidulans | |
Metabisulfite | 0.1 mM, 99% inhibition | Aspergillus nidulans | |
o-phenanthroline hydrate | 3 mM, 30% inhibition | Aspergillus nidulans | |
phenylhydrazine | 1 mM, 86% inhibition, noncompetitive inhibition | Aspergillus nidulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 14 | Hydroquinone monomethylether | range of estimated values for crude or purified extracts | Aspergillus nidulans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aspergillus nidulans | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | 2.1 copper atoms per polypeptide, enzyme form B | Aspergillus nidulans | |
Zn2+ | 0.9 mol per polypeptide | Aspergillus nidulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
x * 48000, enzyme form B, SDS-PAGE | Aspergillus nidulans |
50000 | - |
x * 50000, enzyme form A, SDS-PAGE | Aspergillus nidulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-6-hydroxytryptophan + O2 | Aspergillus nidulans | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nidulans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose, Phenyl Sepharose, enzyme form A and B | Aspergillus nidulans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
conidiophore | - |
Aspergillus nidulans | - |
culture filtrate | - |
Aspergillus nidulans | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme form A, 480.0 DELTA absorbance 470 nm/min/mg, enzyme form B, 258.6 DELTA absorbance 470 nm/min/mg | Aspergillus nidulans |
Storage Stability | Organism |
---|---|
4°C, 3 weeks, no loss of activity | Aspergillus nidulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylcatechol + O2 | - |
Aspergillus nidulans | 4-methyl-o-benzoquinone + H2O | - |
? | |
catechol + 1/2 O2 | - |
Aspergillus nidulans | 1,2-benzoquinone + H2O | - |
? | |
chlorogenic acid + O2 | - |
Aspergillus nidulans | chlorogenoquinone + H2O | - |
? | |
D-catechin + O2 | - |
Aspergillus nidulans | ? | - |
? | |
hydroquinone monomethylether + O2 | - |
Aspergillus nidulans | quinone monomethylether | - |
? | |
N-acetyl-6-hydroxytryptophan + O2 | - |
Aspergillus nidulans | ? | - |
? | |
o-methoxyphenol + O2 + AH2 | trivial name guaiacol | Aspergillus nidulans | 1,2-dihydroxy-3-methoxybenzene + H2O + A | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48000, enzyme form B, SDS-PAGE | Aspergillus nidulans |
? | x * 50000, enzyme form A, SDS-PAGE | Aspergillus nidulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
with hydroquinone monomethylether | Aspergillus nidulans |