Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2,4-pteridinediol | i.e. lumazine, competitively activates the enzyme with respect to ascorbate | Malus domestica | |
bicarbonate | 20 mM bicarbonate pretreatment for 20 min is sufficient to protect and activate the enzyme | Malus domestica | |
cyanide | the enzyme is activated by cyanide concentrations between 0.1 and 1 mM with most efficient activation at 0.5 mM | Malus domestica |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Malus domestica |
Protein Variants | Comment | Organism |
---|---|---|
C133A | the mutant showsincreased activity compared to the wild type enzyme | Malus domestica |
C133P | the mutant shows slightly increased activity compared to the wild type enzyme | Malus domestica |
C165A | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
C28A | the mutant shows increased activity compared to the wild type enzyme | Malus domestica |
E294F | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
E297L | the mutant shows strongly increased activity compared to the wild type enzyme | Malus domestica |
E301D | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
E301L | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
F187Y | the mutant shows increased activity compared to the wild type enzyme | Malus domestica |
F300Q | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
F300Y | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
K144E | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
K158E | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
K158L | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
K158Q | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
K158Q/R175E | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
K158Q/R175Q | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
K158R | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
K158R/R175Q | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
K172E | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
K199E | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
K230E | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
K230Q | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
K230R | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
K292E | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
K292R | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
K296E | the mutant shows increased activity compared to the wild type enzyme | Malus domestica |
N216F | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
P298A | the mutant shows strongly increased activity compared to the wild type enzyme | Malus domestica |
Q188A | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
Q188K | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
Q188N | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
R175A | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
R175E | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
R175E/R244K | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
R175E/S246A | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
R175E/T157A | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
R175G | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
R175H | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
R175K | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
R175Q | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
R244K | the mutant is less active than the native enzyme and has a 5fold higher Km value for 1-aminocyclopropane-1-carboxylate | Malus domestica |
R244K/S246A | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
R244K/S246A/T157A | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
R244K/T157A | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
R299E | inactive | Malus domestica |
R299H | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
R299K | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
R299L | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
S246A | the mutant is less active than the native enzyme and has a 3fold higher Km value for 1-aminocyclopropane-1-carboxylate | Malus domestica |
T157A | the mutation does not affect the Km for 1-aminocyclopropane-1-carboxylate but drastically reduces enzyme activity | Malus domestica |
W203F | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
Y251F | the mutant shows reduced activity compared to the wild type enzyme | Malus domestica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyanide | inhibitory beyond 1 mM | Malus domestica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme E301L, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.031 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme R299L, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.036 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme F300Y, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.046 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme K158R, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.047 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme R299K, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.051 | - |
1-aminocyclopropane-1-carboxylate | wild type enzyme, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.06 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme K158Q, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.062 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme C28A, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.062 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme Y251F, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.063 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme W203F, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.07 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme T157A, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.078 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme K199E, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.083 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme R175K, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.09 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme F187Y, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.094 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme R299H, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.129 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme F300Q, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.139 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme R175H, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.142 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme R175G, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.158 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme N216F, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.173 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme K158E, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.175 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme R244K, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.175 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme R244K/S246A, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.193 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme R175A, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.206 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme K158Q/R175Q, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.218 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme R175Q, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.245 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme R244K/S246A/T157A, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.277 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme S246A, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.279 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme K158R/R175Q, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.281 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme K158L, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.287 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme Q188N, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.379 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme R175E, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
0.659 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme K158Q/R175E, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
1.2 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme R175E/S246A, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica | |
2.3 | - |
1-aminocyclopropane-1-carboxylate | mutant enzyme R175E/T157A, in 50 mM MOPS-HCl (pH 7.2), at 30°C | Malus domestica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-aminocyclopropane-1-carboxylate + ascorbate + O2 | Malus domestica | - |
ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malus domestica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, column chromatography, and gel filtration | Malus domestica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-aminocyclopropane-1-carboxylate + ascorbate + O2 | - |
Malus domestica | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
additional information | the enzyme becomes inactivated by fragmentation and apparently has intrinsic protease and transpeptidase activity | Malus domestica | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1-aminocyclopropane-1-carboxylic acid oxidase | - |
Malus domestica |
ACC oxidase | - |
Malus domestica |
ACCO1 | - |
Malus domestica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | - |
Malus domestica |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the final step in ethylene biosynthesis. The enzyme is involved in the ethylene signal transduction pathway not directly linked to the enzyme reaction | Malus domestica |