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Literature summary for 1.14.17.4 extracted from
- The catalytic mechanism of 1-aminocyclopropane-1-carboxylic acid oxidase (2001), Arch. Biochem. Biophys., 385, 179-185.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Malus domestica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-aminoisobutyric acid | competitive to 1-aminocyclopropane-1-carboxylate | Malus domestica |  |
| D-alanine | competitive to 1-aminocyclopropane-1-carboxylate | Malus domestica | |
| D-alpha-aminobutyric acid | competitive to 1-aminocyclopropane-1-carboxylate | Malus domestica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
1-aminocyclopropane-1-carboxylate | recombinant enzyme, pH 7.0, 30°C | Malus domestica | |
| 14.7 | - |
alpha-aminoisobutyrate | recombinant enzyme, pH 7.0, 30°C | Malus domestica | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-aminocyclopropane-1-carboxylate + ascorbate + O2 | Malus domestica | - |
ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Malus domestica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from Escherichia coli | Malus domestica |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 1-aminocyclopropane-1-carboxylate + ascorbate + O2 = ethene + cyanide + dehydroascorbate + CO2 + 2 H2O | catalytic mechanism | Malus domestica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.003 | - |
at 10 mM L-alpha-aminobutyric acid, purified recombinant enzyme | Malus domestica |
| 0.008 | - |
at 10 mM L-alanine, purified recombinant enzyme | Malus domestica |
| 0.023 | - |
at 10 mM D-alanine, purified recombinant enzyme | Malus domestica |
| 0.051 | - |
at 10 mM alpha-aminoisobutyric acid, purified recombinant enzyme | Malus domestica |
| 0.059 | - |
at 10 mM D-alpha-aminobutyric acid, purified recombinant enzyme | Malus domestica |
| 0.13 | - |
at 10 mM 1-aminocyclopropane-1-carboxylate, purified recombinant enzyme | Malus domestica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-aminocyclopropane-1-carboxylate + ascorbate + O2 | - |
Malus domestica | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
| 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 | stereoselectivity | Malus domestica | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
| 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 | i.e. ACC | Malus domestica | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
| 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 | N-hydroxyl-1-aminocyclopropane-1-carboxylate is no intermediate | Malus domestica | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
| alpha-aminoisobutyric acid + L-ascorbate + O2 | - |
Malus domestica | ammonia + acetone + dehydroascorbate + CO2 + H2O | - |
? | |
| D-alanine + L-ascorbate + O2 | - |
Malus domestica | ? | - |
? | |
| D-alpha-aminobutyric acid + L-ascorbate + O2 | - |
Malus domestica | ammonia + acetone + dehydroascorbate + CO2 + H2O | - |
? | |
| L-alanine + L-ascorbate + O2 | - |
Malus domestica | ? | - |
? | |
| L-alpha-aminobutyric acid + L-ascorbate + O2 | - |
Malus domestica | ammonia + acetone + dehydroascorbate + CO2 + H2O | - |
? | |
| N-hydroxy-alpha-aminoisobutyric acid + L-ascorbate + O2 | - |
Malus domestica | ammonia + acetone + dehydroascorbate + CO2 + H2O | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Malus domestica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
1-aminocyclopropane-1-carboxylate | recombinant enzyme, pH 7.0, 30°C | Malus domestica | |
| 0.07 | - |
alpha-aminoisobutyrate | recombinant enzyme, pH 7.0, 30°C | Malus domestica | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Malus domestica |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.2 | - |
D-alpha-aminobutyric acid | pH 7.0, 30°C | Malus domestica | |
| 7.5 | - |
alpha-aminoisobutyric acid | pH 7.0, 30°C | Malus domestica | |
| 28 | - |
D-alanine | pH7.0, 30°C | Malus domestica | |
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