Cloned (Comment) | Organism |
---|---|
wild-type enzyme and mutants, expression in Escherichia coli BL21(DE3) | Solanum lycopersicum |
Protein Variants | Comment | Organism |
---|---|---|
D179E | site-directed mutagenesis, very low activity, stimulation by bicarbonate | Solanum lycopersicum |
D179N | site-directed mutagenesis, no activity | Solanum lycopersicum |
H177D | site-directed mutagenesis, no activity | Solanum lycopersicum |
H177D/D179E | site-directed mutagenesis, no activity | Solanum lycopersicum |
H177E | site-directed mutagenesis, very low activity, stimulation by bicarbonate | Solanum lycopersicum |
H177Q | site-directed mutagenesis, no activity | Solanum lycopersicum |
H211Q | site-directed mutagenesis, slightly reduced activity | Solanum lycopersicum |
H234D | site-directed mutagenesis, no activity | Solanum lycopersicum |
H234E | site-directed mutagenesis, no activity | Solanum lycopersicum |
H234Q | site-directed mutagenesis, no activity | Solanum lycopersicum |
H39Q | site-directed mutagenesis, slightly reduced activity | Solanum lycopersicum |
H56Q | site-directed mutagenesis, activity similar to the wild-type | Solanum lycopersicum |
H94Q | site-directed mutagenesis, slightly reduced activity | Solanum lycopersicum |
General Stability | Organism |
---|---|
enzyme has a short half-life under catalytic conditions undergoing metal-catalyzed oxidative fragmentation | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00029 | - |
Fe2+ | recombinant mutant H177E, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum | |
0.00043 | - |
Fe2+ | recombinant wild-type enzyme, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum | |
0.00047 | - |
Fe2+ | recombinant mutant H56Q, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum | |
0.0046 | - |
Fe2+ | recombinant mutant D179E, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum | |
0.042 | - |
1-aminocyclopropane-1-carboxylate | recombinant mutant H177E, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum | |
0.083 | - |
1-aminocyclopropane-1-carboxylate | recombinant wild-type enzyme, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum | |
0.121 | - |
1-aminocyclopropane-1-carboxylate | recombinant mutant H56Q, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum | |
0.132 | - |
1-aminocyclopropane-1-carboxylate | recombinant mutant D179E, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Solanum lycopersicum | |
Fe2+ | iron binding ligands are His177, Asp179, His234 | Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-aminocyclopropane-1-carboxylate + ascorbate + O2 | Solanum lycopersicum | biosynthesis of plant signalling molecule ethylene | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type enzyme and mutants from Escherichia coli BL21(DE3) to near homogeneity | Solanum lycopersicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-aminocyclopropane-1-carboxylate + ascorbate + O2 | biosynthesis of plant signalling molecule ethylene | Solanum lycopersicum | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 | i.e. ACC | Solanum lycopersicum | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Solanum lycopersicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00867 | - |
1-aminocyclopropane-1-carboxylate | recombinant mutant D179E, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum | |
0.0095 | - |
1-aminocyclopropane-1-carboxylate | recombinant mutant H177E, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum | |
5.17 | - |
1-aminocyclopropane-1-carboxylate | recombinant mutant H56Q, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum | |
5.9 | - |
1-aminocyclopropane-1-carboxylate | recombinant wild-type enzyme, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Solanum lycopersicum |