Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | absolutely dependent on | Malus domestica | |
ascorbate | minimal 1 mM | Malus domestica | |
CO2 | absolutely dependent on | Malus domestica | |
CO2 | half-maximal activity at 0.5% atmospheric CO2 in vivo or 0.15 mM in the medium in vitro | Malus domestica | |
dithiothreitol | activates in presence of ascorbate, but cannot replace ascorbate | Malus domestica | |
ethylene | activation, in vivo and in vitro | Malus domestica |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Malus domestica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-aminoisobutyric acid | - |
Malus domestica | |
Co2+ | - |
Malus domestica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | absolutely required | Malus domestica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
1 * 35000, SDS-PAGE | Malus domestica |
39000 | - |
gel filtration | Malus domestica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-aminocyclopropane-1-carboxylate + ascorbate + O2 | Malus domestica | absolutely dependent on CO2 | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malus domestica | - |
cv. Golden Delicious | - |
Malus domestica | - |
inducible by ethylene | - |
Purification (Comment) | Organism |
---|---|
180fold to near homogeneity | Malus domestica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | in climacteric stage | Malus domestica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.02 | - |
purified enzyme | Malus domestica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-aminocyclopropane-1-carboxylate + ascorbate + O2 | absolutely dependent on CO2 | Malus domestica | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 | absolutely dependent on CO2 | Malus domestica | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 | i.e. ACC | Malus domestica | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 | absolutely dependent on ascorbate | Malus domestica | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35000, SDS-PAGE | Malus domestica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Malus domestica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Malus domestica |