BRENDA - Enzyme Database
show all sequences of 1.14.16.5

Widespread occurrence of glyceryl ether monooxygenase activity in rat tissues detected by a novel assay

Werner, E.R.; Hermetter, A.; Prast, H.; Golderer, G.; Werner-Felmayer, G.; J. Lipid Res. 48, 1422-1427 (2007) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0026
-
(6R)-tetrahydrobiopterin
pH 8.5, 37°C
Rattus norvegicus
0.0089
-
1-O-pyrenedecyl-sn-glycerol
pH 8.5, 37°C
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Rattus norvegicus
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1-alkyl-sn-glycerol + tetrahydrobiopterin + O2
Rattus norvegicus
-
1-hydroxyalkyl-sn-glycerol + dihydrobiopterin + H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Rattus norvegicus
-
Sprague-Dawley rats
-
Purification (Commentary)
Purification (Commentary)
Organism
partial, 100fold purification of the native enzyme from liver microsomes
Rattus norvegicus
Reaction
Reaction
Commentary
Organism
Reaction ID
1-O-alkyl-sn-glycerol + a 5,6,7,8-tetrahydropteridine + O2 = 1-O-(1-hydroxyalkyl)-sn-glycerol + a 4a-hydroxy-5,6,7,8-tetrahydropteridine
The glyceryl ether substrate is first hydroxylated in a pteridine-dependent monooxygenase reaction to yield the unstable semiacetal product. The enzyme accepts a range of chain lengths from n = 11 to n = 20. The tetrahydrobiopterin cofactor is first converted to a 4alpha-hydroxy derivative and leaves the reaction after dehydration as 6,7[8H]-dihydrobiopterin, which is recycled to tetrahydrobiopterin by dihydropteridine reductase with NADH or NADPH. The semiacetal rearranges to the corresponding aldehyde and glycerol. In the presence of oxygen, the aldehyde is readily oxidized to the corresponding acid
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Rattus norvegicus
-
cerebellum
-
Rattus norvegicus
-
cerebral cortex
-
Rattus norvegicus
-
heart
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
large intestine
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
lung
-
Rattus norvegicus
-
additional information
wide tissue distribution, except female heart, with highest activities in liver, intestine, and cerebellum, overview
Rattus norvegicus
-
ovary
-
Rattus norvegicus
-
skeletal muscle
-
Rattus norvegicus
-
small intestine
-
Rattus norvegicus
-
spleen
-
Rattus norvegicus
-
stomach
-
Rattus norvegicus
-
striatum
-
Rattus norvegicus
-
testis
-
Rattus norvegicus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1-alkyl-sn-glycerol + tetrahydrobiopterin + O2
-
688220
Rattus norvegicus
1-hydroxyalkyl-sn-glycerol + dihydrobiopterin + H2O
-
-
-
?
1-O-pyrenedecyl-sn-glycerol + tetrahydrobiopterin + O2
1-O-pyrenedecyl-sn-glycerol and high-performance liquid chromatographic analysis of reaction mixtures with fluorescence detection, overview
688220
Rattus norvegicus
1-hydroxypyrenedecyl-sn-glycerol + dihydrobiopterin + H2O
further formation of 1-pyrenedecanoic acid
-
-
?
Synonyms
Synonyms
Commentary
Organism
glyceryl ether monooxygenase
-
Rattus norvegicus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
assay at
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
(6R)-tetrahydrobiopterin
strictly dependent on, stereospecific, overview
Rattus norvegicus
(6RS)-6,7-dimethyltetrahydropterin
94.2% of the activity with 6R-tetrahydrobiopterin
Rattus norvegicus
(6RS)-6-hydroxymethyltetrahydropterin
16% of the activity with 6R-tetrahydrobiopterin
Rattus norvegicus
(6RS)-6-methyltetrahydropterin
60.9% of the activity with 6R-tetrahydrobiopterin
Rattus norvegicus
(6RS)-tetrahydrodictyopterin
44.7% of the activity with 6R-tetrahydrobiopterin
Rattus norvegicus
(6RS)-tetrahydroneopterin
124% of the activity with 6R-tetrahydrobiopterin
Rattus norvegicus
(6S)-tetrahydrobiopterin
3.5% of the activity with 6R-tetrahydrobiopterin
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
(6R)-tetrahydrobiopterin
strictly dependent on, stereospecific, overview
Rattus norvegicus
(6RS)-6,7-dimethyltetrahydropterin
94.2% of the activity with 6R-tetrahydrobiopterin
Rattus norvegicus
(6RS)-6-hydroxymethyltetrahydropterin
16% of the activity with 6R-tetrahydrobiopterin
Rattus norvegicus
(6RS)-6-methyltetrahydropterin
60.9% of the activity with 6R-tetrahydrobiopterin
Rattus norvegicus
(6RS)-tetrahydrodictyopterin
44.7% of the activity with 6R-tetrahydrobiopterin
Rattus norvegicus
(6RS)-tetrahydroneopterin
124% of the activity with 6R-tetrahydrobiopterin
Rattus norvegicus
(6S)-tetrahydrobiopterin
3.5% of the activity with 6R-tetrahydrobiopterin
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0026
-
(6R)-tetrahydrobiopterin
pH 8.5, 37°C
Rattus norvegicus
0.0089
-
1-O-pyrenedecyl-sn-glycerol
pH 8.5, 37°C
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Rattus norvegicus
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1-alkyl-sn-glycerol + tetrahydrobiopterin + O2
Rattus norvegicus
-
1-hydroxyalkyl-sn-glycerol + dihydrobiopterin + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial, 100fold purification of the native enzyme from liver microsomes
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Rattus norvegicus
-
cerebellum
-
Rattus norvegicus
-
cerebral cortex
-
Rattus norvegicus
-
heart
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
large intestine
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
lung
-
Rattus norvegicus
-
additional information
wide tissue distribution, except female heart, with highest activities in liver, intestine, and cerebellum, overview
Rattus norvegicus
-
ovary
-
Rattus norvegicus
-
skeletal muscle
-
Rattus norvegicus
-
small intestine
-
Rattus norvegicus
-
spleen
-
Rattus norvegicus
-
stomach
-
Rattus norvegicus
-
striatum
-
Rattus norvegicus
-
testis
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1-alkyl-sn-glycerol + tetrahydrobiopterin + O2
-
688220
Rattus norvegicus
1-hydroxyalkyl-sn-glycerol + dihydrobiopterin + H2O
-
-
-
?
1-O-pyrenedecyl-sn-glycerol + tetrahydrobiopterin + O2
1-O-pyrenedecyl-sn-glycerol and high-performance liquid chromatographic analysis of reaction mixtures with fluorescence detection, overview
688220
Rattus norvegicus
1-hydroxypyrenedecyl-sn-glycerol + dihydrobiopterin + H2O
further formation of 1-pyrenedecanoic acid
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
assay at
Rattus norvegicus
Other publictions for EC 1.14.16.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745497
Marquet
Exome sequencing identifies t ...
Homo sapiens
J. Infect. Dis.
216
22-28
2017
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2
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1
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6
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2
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1
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1
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1
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745840
de Rond
Oxidative cyclization of prod ...
Pseudoalteromonas rubra
Nat. Chem. Biol.
13
1155-1157
2017
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1
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1
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-
2
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1
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4
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1
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1
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745589
Alrayes
The alkylglycerol monooxygena ...
Homo sapiens
J. Neurol. Sci.
363
240-244
2016
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1
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1
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5
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1
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746303
Watschinger
Tetrahydrobiopterin and alkyl ...
Mus musculus
Proc. Natl. Acad. Sci. USA
112
2431-2436
2015
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-
-
-
-
-
-
-
-
-
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1
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6
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5
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1
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1
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1
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5
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1
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-
-
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-
-
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-
726881
Tokuoka
Alkylglycerol monooxygenase as ...
Mus musculus
Biochem. Biophys. Res. Commun.
436
306-312
2013
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-
1
-
2
-
-
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1
-
1
2
-
3
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2
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2
1
2
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1
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1
1
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2
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1
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1
2
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2
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2
1
-
-
-
-
-
-
-
-
1
1
1
1
-
-
727650
Watschinger
Alkylglycerol monooxygenase ...
Danio rerio, Caenorhabditis elegans, no activity in Drosophila melanogaster, Rattus norvegicus
IUBMB Life
65
366-372
2013
-
-
1
-
-
-
-
-
1
3
3
6
-
12
-
-
1
-
-
1
-
-
13
3
3
-
-
-
-
-
-
-
3
-
-
-
-
-
1
3
-
-
-
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1
3
3
6
-
-
-
1
-
1
-
-
13
3
-
-
-
-
-
-
-
-
-
1
1
-
-
-
726903
Watschinger
Catalytic residues and a predi ...
Homo sapiens
Biochem. J.
443
279-286
2012
-
-
1
-
18
-
1
2
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1
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1
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1
1
1
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1
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2
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1
1
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18
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2
1
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2
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1
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1
1
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716754
Watschinger
Identification of the gene enc ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
107
13672-13677
2010
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1
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1
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1
2
1
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14
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2
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1
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1
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2
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696542
Watschinger
Glyceryl ether monooxygenase r ...
Rattus norvegicus
Biol. Chem.
390
3-10
2009
-
-
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7
-
2
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1
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1
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1
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1
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3
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1
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1
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1
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1
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7
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2
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1
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1
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1
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3
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1
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-
-
-
-
-
-
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-
688220
Werner
Widespread occurrence of glyce ...
Rattus norvegicus
J. Lipid Res.
48
1422-1427
2007
-
-
-
-
-
-
-
2
1
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1
-
4
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1
1
-
16
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2
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1
1
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1
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7
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7
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2
1
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1
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1
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16
-
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2
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1
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1
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-
438648
Taguchi
Glyceryl-ether monooxygenase [ ...
Canis lupus familiaris, Cavia porcellus, Oryctolagus cuniculus, Meriones sp., Mesocricetus auratus, Mus musculus, Rattus norvegicus
Med. Res. Rev.
18
43-89
1998
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20
23
1
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10
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1
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7
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1
49
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6
5
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6
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21
5
23
1
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7
-
1
49
-
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-
-
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438652
Taguchi
-
Glyceryl-ether monooxygenase [ ...
Rattus norvegicus
J. Chem. Soc. Perkin Trans. I
1997
303-307
1997
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1
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1
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2
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-
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438645
Armarego
-
Glyceryl-ether monooxygenase [ ...
Rattus norvegicus
Pteridines
7
90
1996
-
-
-
-
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1
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1
-
1
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1
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5
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1
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1
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1
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1
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1
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5
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-
-
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438650
Taguchi
-
Glyceryl-ether monooxygenase [ ...
Rattus norvegicus
Pteridines
6
45-57
1995
-
-
-
-
-
-
-
1
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1
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6
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1
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6
-
-
-
-
-
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-
-
-
-
-
438646
Kosar-Hashemi
-
Glyceryl-ether monooxygenase [ ...
Rattus norvegicus
Pteridines
5
1-7
1994
-
-
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-
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1
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1
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1
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438647
Kurisu
-
Glyceryl-ether monooxygenase [ ...
Rattus norvegicus
Pteridines
5
95-101
1994
-
-
-
-
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-
4
1
1
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-
1
-
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1
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1
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4
-
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4
4
1
1
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438653
Taguchi
Glyceryl-ether monooxygenase ( ...
Rattus norvegicus
Biol. Chem. Hoppe-Seyler
375
329-334
1994
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2
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438649
Kosar-Hashemi
Glyceryl ether monooxygenase [ ...
Rattus norvegicus
Adv. Exp. Med. Biol.
338
93-96
1993
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438651
Kosar-Hashemi
A convenient spectrophotometri ...
Rattus norvegicus
Biol. Chem. Hoppe-Seyler
374
9-25
1993
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11
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17
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17
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438643
Ishibashi
Affinity purification of alkyl ...
Rattus norvegicus
J. Lipid Res.
26
393-395
1985
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7
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438640
Ishibashi
Solubilization and partial cha ...
Rattus norvegicus
Eur. J. Biochem.
132
23-27
1983
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1
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438644
Rock
Stimulation of the microsomal ...
Rattus norvegicus
Biochim. Biophys. Acta
450
469-473
1976
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438642
Snyder
Tetrahydropteridine-dependent ...
Rattus norvegicus
Biochim. Biophys. Acta
316
259-265
1973
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438639
Soodsma
Partial characterization of th ...
Rattus norvegicus
J. Biol. Chem.
247
3923-3929
1972
3
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8
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438641
Pfleger
The biocleavage of isomeric gl ...
Canis lupus familiaris, Cavia porcellus, Oryctolagus cuniculus, Limax maximus, Meriones sp., Mesocricetus auratus, Mus musculus, Rattus norvegicus
Biochim. Biophys. Acta
144
633-648
1967
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