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Literature summary for 1.14.16.4 extracted from

  • Kurosawa, N.; Hirata, T.; Suzuki, H.
    Characterization of putative tryptophan monooxygenase from Ralstonia solanacearum (2009), J. Biochem., 146, 23-32.
    View publication on PubMed
Search for more literature for 1.14.16.4

Activating Compound

Activating Compound Comment Organism Structure
additional information pronase is most effective for activation. Trypsin, chymotrypsin, V8 protease and lysylendoprotease also activate PTMO, but the activity obtained using these proteases is only 5% of that with pronase Ralstonia solanacearum

Cloned(Commentary)

Cloned (Comment) Organism
pBR322 containing the open reading frame of PTMO, PCR products inserted into the EcoRV site of pBluescript vector, expressed from vector pET 22b(+) in Escherichia coli BL21(DE3) Ralstonia solanacearum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
162000
-
 gel filtration Ralstonia solanacearum

Organism

Organism UniProt Comment Textmining
Ralstonia solanacearum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
on Ni column Ralstonia solanacearum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine + ? + O2 relative high activity with pronase-treated PTMO Ralstonia solanacearum ?
-
 ?
L-phenylalanine + tetrahydropteridine + O2 purified PTMO shows no activity, relative high activity with pronase-treated PTMO Ralstonia solanacearum L-tyrosine + dihydropteridine + H2O
-
 ?
L-tryptophan + tetrahydrobiopterin + O2 relative high activity with pronase-treated PTMO Ralstonia solanacearum 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
-
 ?
L-tyrosine + tetrahydropteridine + O2 relative high activity with pronase-treated PTMO Ralstonia solanacearum ?
-
 ?
additional information pronase-treated PTMO shows less than 5% activity of that of L-phenylalanine for L-lysine, L-histidine and L-threonine Ralstonia solanacearum ?
-
 ?

Synonyms

Synonyms Comment Organism
PTMO
-
 Ralstonia solanacearum
putative tryptophan monooxygenase
-
 Ralstonia solanacearum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Ralstonia solanacearum

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7.5
-
 Ralstonia solanacearum

Cofactor

Cofactor Comment Organism Structure
tetrahydrobiopterin
-
 Ralstonia solanacearum