Any feedback?
Literature summary for 1.14.16.4 extracted from
- Influence of human tryptophan hydroxylase 2 N- and C-terminus on enzymatic activity and oligomerization (2007), J. Neurochem., 102, 1887-1894.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DTT | - |
Homo sapiens |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type isozyme TPH1 in COS7 cells | Homo sapiens |
| expression of wild-type isozyme TPH2, and of TPH2 deletion mutants in COS7 cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of various deletion mutants of TPH2: DN44, DC17, DC19,and DC51. Removal of 44 amino acids from the N-terminus of TPH2 results in a 3-4fold increased Vmax, which indicates a strong inhibitory function of this part on the enzymes activity. Deletion mutants DC17, DC19and DC51, which lack the putative C-terminal leucine zipper tetramerization domain, exist as monomeric enzymes | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | removal of 44 amino acids from the N-terminus of TPH2 results in a 3-4fold increased Vmax, which indicates a strong inhibitory function of this part on the enzymes activity | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of wild-type isozyme TPH1, overview | Homo sapiens | |
| additional information | - |
additional information | kinetics of wild-type isozyme TPH2 and of TPH2 deletion mutants, overview | Homo sapiens | |
| 0.015 | - |
tetrahydrobiopterin | pH 7.0, 30°C, recombinant isozyme TPH1 | Homo sapiens | |
| 0.053 | - |
6-methyl-tetrahydropterin | pH 7.0, 30°C, recombinant isozyme TPH1 | Homo sapiens | |
| 0.077 | - |
tetrahydrobiopterin | pH 7.0, 30°C, recombinant isozyme TPH2 | Homo sapiens | |
| 0.802 | - |
6-methyl-tetrahydropterin | pH 7.0, 30°C, recombinant isozyme TPH2 | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-tryptophan + tetrahydrobiopterin + O2 | Homo sapiens | first and rate limiting step in the biosynthesis of the neurotransmitter serotonin | 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
isozyme TPH1 | - |
| Homo sapiens | - |
isozyme TPH2 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-tryptophan + 6-methyl-tetrahydropterin + O2 | - |
Homo sapiens | 5-hydroxy-L-tryptophan + 6-methyl-4a-hydroxytetrahydropterin | - |
? | |
| L-tryptophan + tetrahydrobiopterin + O2 | - |
Homo sapiens | 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin | - |
? | |
| L-tryptophan + tetrahydrobiopterin + O2 | first and rate limiting step in the biosynthesis of the neurotransmitter serotonin | Homo sapiens | 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TPH1 | - |
Homo sapiens |
| TPH2 | - |
Homo sapiens |
| tryptophan hydroxylase 1 | - |
Homo sapiens |
| tryptophan hydroxylase 2 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
