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Literature summary for 1.14.16.2 extracted from

  • Daubner, S.C.; Fitzpatrick, P.F.
    Mutation to phenylalanine of tyrosine 371 in tyrosine hydroxylase increases the affinity for phenylalanine (1998), Biochemistry, 37, 16440-16444.
    View publication on PubMed
Search for more literature for 1.14.16.2

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
Y371F increased Km for tyrosine and pterin cosubstrates, highly decreased Km for phenylalanine Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 phenylalanine recombinant mutant Y371F Rattus norvegicus
0.027
-
 Tetrahydropterin recombinant wild-type, pH 7.1 Rattus norvegicus
0.032
-
 tyrosine recombinant wild-type, pH 6.0 Rattus norvegicus
0.033
-
 6-Methyl-5,6,7,8-tetrahydropterin recombinant wild-type, pH 7.1 Rattus norvegicus
0.051
-
 tyrosine recombinant wild-type, pH 7.1 Rattus norvegicus
0.053
-
 Tetrahydropterin recombinant mutant Y371F, pH 7.1 Rattus norvegicus
0.055
-
 6-Methyl-5,6,7,8-tetrahydropterin recombinant wild-type, pH 6.0 Rattus norvegicus
0.059
-
 6-Methyl-5,6,7,8-tetrahydropterin recombinant mutant Y371F, pH 7.1 Rattus norvegicus
0.065
-
 tyrosine recombinant mutant Y371F, pH 7.1 Rattus norvegicus
0.065
-
 6-Methyl-5,6,7,8-tetrahydropterin recombinant mutant Y371F, pH 6.0 Rattus norvegicus
0.071
-
 tyrosine recombinant mutant Y371F, pH 6.0 Rattus norvegicus
0.11
-
 phenylalanine recombinant wild-type Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron
-
 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P04177
-
-

Reaction

Reaction Comment Organism Reaction ID
L-tyrosine + a 5,6,7,8-tetrahydropteridine + O2 = L-dopa + a 4a-hydroxy-5,6,7,8-tetrahydropteridine mechanism Rattus norvegicus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + tetrahydropteridine + O2 recombinant wild-type and mutant Rattus norvegicus L-tyrosine + dihydropteridine + H2O
-
 ?
L-tyrosine + tetrahydrobiopterin + O2 recombinant wild-type and mutant Rattus norvegicus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O 3,4-dihydroxy-L-phenylalanine is identical with dopa ?

Synonyms

Synonyms Comment Organism
TH
-
 Rattus norvegicus