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Literature summary for 1.14.16.2 extracted from
- L-DOPA is a substrate for tyrosine hydroxylase (1997), J. Neurochem., 69, 1720-1728.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | inhibitory effect of several compounds on L-dopa-oxidase activity | Homo sapiens | |
| additional information | activation by phosphorylation | Homo sapiens | |
| additional information | activation by phosphorylation | Rattus norvegicus | |
| additional information | activation by phosphorylation | Bos taurus | |
| thiols | activate the L-dopa activity | Homo sapiens |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| 4 isoforms, overexpression in Escherichia coli, phosphorylation-free | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Bos taurus | |
| 1,10-phenanthroline | complete | Homo sapiens | |
| 3-iodo-L-tyrosine | - |
Bos taurus | |
| 3-iodo-L-tyrosine | complete | Homo sapiens | |
| Bathocuproine sulfonate | slightly | Homo sapiens | |
| bathophenanthroline sulfonate | - |
Bos taurus | |
| bathophenanthroline sulfonate | - |
Homo sapiens | |
| dihydrobiopterin | L-dopa-oxidase activity | Homo sapiens | |
| L-Dopa | - |
Bos taurus | |
| L-Dopa | above 0.15 mM inhibiting L-dopa-oxidase activity in presence of tetrahydropterin, but not with 6,7-dimethyltetrahydropterin | Homo sapiens | |
| L-Dopa | - |
Rattus norvegicus | |
| noradrenaline | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
Tetrahydropterin | recombinant phosphorylated hTH1, L-dopa-oxidase activity | Homo sapiens | |
| 0.056 | - |
L-Dopa | recombinant hTH1, dopa-oxidase activity with tetrahydropterin | Homo sapiens | |
| 0.146 | - |
L-Dopa | recombinant, dopa-oxidase activity with 6,7-dimethyltetrahydropterin | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | slightly activating | Homo sapiens | |
| Iron | - |
Rattus norvegicus | |
| Iron | required | Homo sapiens | |
| Iron | Fe2+ activates | Homo sapiens | |
| Iron | Fe2+ activates | Bos taurus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Homo sapiens | - |
4 isoforms hTH1, hTH2, hTH3, hTH4 | - |
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | - |
Rattus norvegicus | - |
| adrenal gland | - |
Bos taurus | - |
| pheochromocytoma cell | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
dopa-oxidase activity of the 4 isoforms | Homo sapiens |
| 0.16 | - |
purified recombinant isoform hTH3 | Homo sapiens |
| 0.2 | - |
purified enzyme | Rattus norvegicus |
| 0.2 | - |
purified recombinant isoform hTH4 | Homo sapiens |
| 0.29 | - |
purified recombinant isoform hTH2 | Homo sapiens |
| 0.33 | - |
purified recombinant isoform hTH1, tyrosine concentration is 0.2 mM | Homo sapiens |
| 0.79 | - |
purified recombinant isoform hTH1, tyrosine concentration is 0.05 mM | Homo sapiens |
| 2.5 | - |
purified enzyme, tyrosine concentration is 0.2 mM | Bos taurus |
| 20.7 | - |
purified enzyme, tyrosine concentration is 0.05 mM | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dihydroxy-L-phenylalanine + 6,7-dimethyltetrahydropteridine + O2 | i.e. L-dopa | Homo sapiens | ? | - |
? | |
| 3,4-dihydroxy-L-phenylalanine + 6,7-dimethyltetrahydropteridine + O2 | L-dopa-oxidase activity | Homo sapiens | ? | - |
? | |
| 3,4-dihydroxy-L-phenylalanine + tetrahydropterin + O2 | i.e. L-dopa | Homo sapiens | ? | - |
? | |
| 3,4-dihydroxy-L-phenylalanine + tetrahydropterin + O2 | 4 isoforms, thiols required, stimulation by Fe2+ and tetrahydropterin | Homo sapiens | ? | - |
? | |
| L-tyrosine + tetrahydrobiopterin + O2 | - |
Homo sapiens | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | 3,4-dihydroxy-L-phenylalanine is identical with dopa | ? | |
| L-tyrosine + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | 3,4-dihydroxy-L-phenylalanine is identical with dopa | ? | |
| L-tyrosine + tetrahydrobiopterin + O2 | - |
Bos taurus | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | 3,4-dihydroxy-L-phenylalanine is identical with dopa | ? | |
| additional information | - |
Rattus norvegicus | ? | - |
? | |
| additional information | under aerobic conditions can generate significant amounts of reactive oxygen species, including hydrogen peroxide and hydroxyl radicals | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TH | - |
Homo sapiens |
| TH | - |
Rattus norvegicus |
| tyrosine-3-monooxygenase | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Rattus norvegicus |
| 7 | - |
recombinant enzyme isoforms, L-dopa-oxidase activity | Homo sapiens |
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