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Literature summary for 1.14.16.2 extracted from

  • Fujisawa, H.; Okuno, S.
    Tyrosine 3-monooxygenase from rat adrenals (1987), Methods Enzymol., 142, 63-71.
    View publication on PubMed
Search for more literature for 1.14.16.2

General Stability

General Stability Organism
EDTA, 0.1 mM stabilizes Rattus norvegicus
glycerol, 25% stabilizes Rattus norvegicus
Tween 80, 0.05% stabilizes Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Catecholamines
-
 Rattus norvegicus
dopamine
-
 Rattus norvegicus
epinephrine
-
 Rattus norvegicus
norepinephrine
-
 Rattus norvegicus
O2
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.058
-
 2-amino-4-hydroxy-6-methyl-5,6,7,8-tetrahydropteridine
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 4 * 59000, SDS-PAGE Rattus norvegicus
260000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tyrosine + (6R)-L-erythro-1',2'-dihydroxypropyltetrahydropterin + O2 Rattus norvegicus first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine ?
-
 ?
L-tyrosine + tetrahydrobiopterin + O2 Rattus norvegicus first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal medulla
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1604
-
 purified enzyme Rattus norvegicus

Storage Stability

Storage Stability Organism
-80°C, 0.05% Tween 80, 1 mM EDTA, 25% glycerol, 3 months Rattus norvegicus
4°C, 24 h, 0.05% Tween 80, 1 mM EDTA, 25% glycerol, 68% loss of activity Rattus norvegicus
4°C, 5 h, without stabilizing agents, almost complete loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine + (6R)-L-erythro-1',2'-dihydroxypropyltetrahydropterin + O2 first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine Rattus norvegicus ?
-
 ?
L-tyrosine + 2-amino-4-hydroxy-6,7-dimethyltetrahydropterin + O2 synthetic pterin as electron donor Rattus norvegicus ?
-
 ?
L-tyrosine + 2-amino-4-hydroxy-6-methyltetrahydropterin + O2 synthetic pterin as electron donor Rattus norvegicus ?
-
 ?
L-tyrosine + tetrahydrobiopterin + O2
-
 Rattus norvegicus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O 3,4-dihydroxy-L-phenylalanine is identical with dopa ?
L-tyrosine + tetrahydrobiopterin + O2 first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine Rattus norvegicus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 59000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pH profile is affected by a variety of conditions: enzymatic phosphorylation by cAMP-dependent protein kinase, calmodulin-dependent protein kinase II and presence of polyanions Rattus norvegicus
5.4 5.5 crude extract Rattus norvegicus
6 7.5 purified enzyme Rattus norvegicus