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Literature summary for 1.14.16.2 extracted from

  • Nagatsu, T.; Oka, K.
    Tyrosine 3-monooxygenase from bovine adrenal medulla (1987), Methods Enzymol., 142, 56-62.
    View publication on PubMed
Search for more literature for 1.14.16.2

Inhibitors

Inhibitors Comment Organism Structure
3-iodo-L-tyrosine
-
 Bos taurus
tetrahydrobiopterin substrate inhibition at tyrosine and O2 concentrations higher than 0.1 mM and 2.2 mM, respectively Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km dependence on substrate concentrations Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane particle-bound Bos taurus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 4 * 60000, SDS-PAGE Bos taurus
280000
-
 gel filtration Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from adrenal medulla Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal medulla
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.88
-
 purified enzyme Bos taurus

Storage Stability

Storage Stability Organism
-80°C, protein concentration above 0.05 mg/ml, 2 months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine + (6R)-L-erythro-tetrahydrobiopterin + O2 preferred electron donor Bos taurus ?
-
 ?
L-tyrosine + 2-amino-4-hydroxy-6,7-dimethyltetrahydropterin + O2
-
 Bos taurus ?
-
 ?
L-tyrosine + 2-amino-4-hydroxy-6-methyltetrahydropterin + O2
-
 Bos taurus ?
-
 ?
L-tyrosine + 6-methyltetrahydropterin + O2 artificial cosubstrate Bos taurus ?
-
 ?
L-tyrosine + tetrahydrobiopterin + O2 substrate inhibition occurs at 0.1 mM tyrosine or O2 with (6R)- and (6RS)-tetrahydrobiopterin Bos taurus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O 3,4-dihydroxy-L-phenylalanine is identical with dopa ?

Subunits

Subunits Comment Organism
tetramer 4 * 60000, SDS-PAGE Bos taurus