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Literature summary for 1.14.16.1 extracted from

  • Boonyapiwat, B.; Forbes, B.; Mitchell, S.; Steventon, G.B.
    Phenylalanine 4-monooxygenase and the S-oxidation of S-carboxymethyl-L-cysteine by human cytosolic fractions (2008), Drug Metabol. Drug Interact., 23, 261-282.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl 99.0% inhibition at 1.0 mM using L-phenylalanine as substrate, 99.0% inhibition at 1.0 mM using S-carboxymethyl-L-cysteine as substrate Homo sapiens
3-iodotyrosine 3.0% inhibition at 1.0 mM using L-phenylalanine as substrate, 5.2% inhibition at 1.0 mM using S-carboxymethyl-L-cysteine as substrate Homo sapiens
4-Chlorophenylalanine specific PAH inhibitor, 97.2% inhibition at 1.0 mM using L-phenylalanine as substrate, 99.2% inhibition at 1.0 mM using S-carboxymethyl-L-cysteine as substrate Homo sapiens
6-Fluorotryptophan 2.5% inhibition at 1.0 mM using L-phenylalanine as substrate, 4.5% inhibition at 1.0 mM using S-carboxymethyl-L-cysteine as substrate Homo sapiens
L-phenylalanine competitive inhibition of the S-oxidation of S-carboxymethyl-L-cysteine, 92.8% inhibition at 1.0 mM using S-carboxymethyl-L-cysteine as substrate Homo sapiens
additional information not inhibited by the nitric oxide synthase inhibitors N(G)-monomethyl-D-arginine, 7-nitroindazole, L-thiocitrulline, N(G)-monomethyl-L-arginine, (S)-methy-L-thiocitrulline, and L-N5-(1-iminoethyl)ornithine; PH8, the large aromatic amino acid hydroxylase monoclonal antibody, inhibits the production of both L-tyrosine and S-carboxymethyl-L-cysteine S-oxide to 99.0% and 99.3% at 0.2 mg/ml concentration Homo sapiens
S-carboxymethyl-L-cysteine competitive inhibitor of the aromatic C-oxidation of L-phenylalanine, 25.2% inhibition at 5.0 mM using L-phenylalanine as substrate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0231
-
5,6,7,8-tetrahydrobiopterin using L-phenylalanine as cosubstrate, in pooled hepatic cytosolic enzyme fraction, at 37°C Homo sapiens
0.02333
-
5,6,7,8-tetrahydrobiopterin using L-phenylalanine as cosubstrate, in pooled hepatic cytosolic enzyme fraction activated with 1 mM lysophosphatidylcholine, at 37°C Homo sapiens
0.07312
-
5,6,7,8-tetrahydrobiopterin using S-carboxymethyl-L-cysteine as cosubstrate, in pooled hepatic cytosolic enzyme fraction activated with 1 mM lysophosphatidylcholine, at 37°C Homo sapiens
0.07812
-
5,6,7,8-tetrahydrobiopterin using S-carboxymethyl-L-cysteine as cosubstrate, in pooled hepatic cytosolic enzyme fraction, at 37°C Homo sapiens
0.16
-
L-phenylalanine in pooled hepatic cytosolic enzyme fraction activated with 1 mM lysophosphatidylcholine, at 37°C Homo sapiens
3.05
-
L-phenylalanine in pooled hepatic cytosolic enzyme fraction, at 37°C Homo sapiens
16.22
-
S-carboxymethyl-L-cysteine
-
Homo sapiens
16.53
-
S-carboxymethyl-L-cysteine in pooled hepatic cytosolic enzyme fraction activated with 1 mM lysophosphatidylcholine, at 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + 5,6,7,8-tetrahydrobiopterin + O2
-
Homo sapiens L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
?
S-carboxymethyl-L-cysteine + 5,6,7,8-tetrahydrobiopterin + O2
-
Homo sapiens S-carboxymethyl-L-cysteine S-oxide + dihydrobiopterin + H2O
-
?

Synonyms

Synonyms Comment Organism
PAH
-
Homo sapiens
phenylalanine hydroxylase
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
5,6,7,8-tetrahydro-L-biopterin
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
17.23
-
S-carboxymethyl-L-cysteine in pooled hepatic cytosolic enzyme fraction, at 37°C Homo sapiens