Protein Variants | Comment | Organism |
---|---|---|
C237A | increase of basal activity and affinity for substrate L-phenylalanine | Homo sapiens |
C237D | increase of basal activity and affinity for substrate L-phenylalanine | Homo sapiens |
C237R | reduced activity, elimination of positive cooperativity | Homo sapiens |
R68A | increase of basal activity and affinity for substrate L-phenylalanine | Homo sapiens |
R68V | little decrease in activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
tetrahydrobiopterin | mutant C237R | Homo sapiens | |
0.034 | - |
tetrahydrobiopterin | mutant C237A | Homo sapiens | |
0.034 | - |
tetrahydrobiopterin | mutant C237D | Homo sapiens | |
0.035 | - |
tetrahydrobiopterin | wild-type | Homo sapiens | |
0.037 | - |
tetrahydrobiopterin | mutant R68A | Homo sapiens | |
0.037 | - |
tetrahydrobiopterin | mutant R68V | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-phenylalanine + a 5,6,7,8-tetrahydropteridine + O2 = L-tyrosine + a 4a-hydroxy-5,6,7,8-tetrahydropteridine | analysis of conformational changes upon substrate binding | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.77 | - |
mutant R68V, preincubation with L-phenylalanine | Homo sapiens |
1.08 | - |
mutant C237R | Homo sapiens |
1.1 | - |
wild-type, preincubation with L-phenylalanine | Homo sapiens |
1.13 | - |
mutant C237R, preincubation with L-phenylalanine | Homo sapiens |
2.14 | - |
mutant R68V | Homo sapiens |
2.2 | - |
mutant R68A, preincubation with L-phenylalanine | Homo sapiens |
2.22 | - |
mutant C237A, preincubation with L-phenylalanine | Homo sapiens |
2.94 | - |
mutant R68A | Homo sapiens |
2.97 | - |
mutant C237A | Homo sapiens |
3.25 | - |
wild-type | Homo sapiens |
3.81 | - |
mutant C237D | Homo sapiens |
4.19 | - |
mutant C237D, preincubation with L-phenylalanine | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + tetrahydrobiopterin + O2 | - |
Homo sapiens | L-tyrosine + dihydrobiopterin + H2O | - |
? |