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Literature summary for 1.14.16.1 extracted from

  • Thorolfsson, M.; Teigen, K.; Martinez, A.
    Activation of phenylalanine hydroxylase: effect of substitutions at Arg68 and Cys237 (2003), Biochemistry, 42, 3419-3428.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
C237A increase of basal activity and affinity for substrate L-phenylalanine Homo sapiens
C237D increase of basal activity and affinity for substrate L-phenylalanine Homo sapiens
C237R reduced activity, elimination of positive cooperativity Homo sapiens
R68A increase of basal activity and affinity for substrate L-phenylalanine Homo sapiens
R68V little decrease in activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
tetrahydrobiopterin mutant C237R Homo sapiens
0.034
-
tetrahydrobiopterin mutant C237A Homo sapiens
0.034
-
tetrahydrobiopterin mutant C237D Homo sapiens
0.035
-
tetrahydrobiopterin wild-type Homo sapiens
0.037
-
tetrahydrobiopterin mutant R68A Homo sapiens
0.037
-
tetrahydrobiopterin mutant R68V Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
L-phenylalanine + a 5,6,7,8-tetrahydropteridine + O2 = L-tyrosine + a 4a-hydroxy-5,6,7,8-tetrahydropteridine analysis of conformational changes upon substrate binding Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.77
-
mutant R68V, preincubation with L-phenylalanine Homo sapiens
1.08
-
mutant C237R Homo sapiens
1.1
-
wild-type, preincubation with L-phenylalanine Homo sapiens
1.13
-
mutant C237R, preincubation with L-phenylalanine Homo sapiens
2.14
-
mutant R68V Homo sapiens
2.2
-
mutant R68A, preincubation with L-phenylalanine Homo sapiens
2.22
-
mutant C237A, preincubation with L-phenylalanine Homo sapiens
2.94
-
mutant R68A Homo sapiens
2.97
-
mutant C237A Homo sapiens
3.25
-
wild-type Homo sapiens
3.81
-
mutant C237D Homo sapiens
4.19
-
mutant C237D, preincubation with L-phenylalanine Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + tetrahydrobiopterin + O2
-
Homo sapiens L-tyrosine + dihydrobiopterin + H2O
-
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