Cloned (Comment) | Organism |
---|---|
expression of wild-type, L348V, L349L and V388M mutant enzyme maltose-binding-protein fusions in Escherichia coli and COS cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
L348V | 25% activity after expression in Escherichia coli in the absence of GroESL, 55% in the presence of GroESL, 77% activity after expression in COS cells at 27°C | Homo sapiens |
S348L | instable enzyme forming aggregates after expression in Escherichia coli in the presence of GroESL | Homo sapiens |
V388M | 40% activity after expression in Escherichia coli in the absence of GroESL, 82% in the presence of GroESL, 78% activity after expression in COS cells at 27°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00439 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type, L348V and V388M mutant enzyme, affinity chromatography | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
V388M mutant enzyme, 50% activity after 10 min | Homo sapiens |
51 | - |
L348V mutant enzyme, 50% activity after 10 min | Homo sapiens |
59 | - |
recombinant wild-type enzyme, 50% activity after 10 min | Homo sapiens |