Any feedback?
Literature summary for 1.14.16.1 extracted from
- Recombinant human phenylalanine hydroxylase: novel regulatory and structural properties (1996), Arch. Biochem. Biophys., 333, 85-95.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cAMP-dependent protein kinase | - |
Rattus norvegicus | |
| cAMP-dependent protein kinase | 1.5fold stimulation of recombinant enzyme | Homo sapiens | |
| lysolecithin | - |
Rattus norvegicus |  |
| lysolecithin | 2fold activation of recombinant enzyme, cofactor tetrahydrobiopterin | Homo sapiens | |
| phenylalanine | - |
Rattus norvegicus | |
| phenylalanine | recombinant enzyme, 2fold increase in activity, cofactor tetrahydrobiopterin | Homo sapiens | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (7R)-5,6,7,8-tetrahydrobiopterin | - |
Homo sapiens | |
| (7R)-5,6,7,8-tetrahydropterin | 0.001 mM, 50% inhibition at 0.5 mM phenylalanine, 0.004 mM, 50% inhibition at 0.1 mM phenylalanine, recombinant enzyme | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
(6R)-5,6,7,8-tetrahydrobiopterin | recombinant enzyme | Homo sapiens | |
| 0.025 | - |
(6R)-5,6,7,8-tetrahydrobiopterin | recombinant enzyme, phenylalanine-activated | Homo sapiens | |
| 0.043 | - |
phenylalanine | recombinant enzyme, cofactor (6R)-methyltetrahydropterin | Homo sapiens | |
| 0.045 | - |
6-methyltetrahydropterin | recombinant enzyme | Homo sapiens | |
| 0.049 | - |
phenylalanine | phenylalanine-activated recombinant enzyme, cofactor tetrahydrobiopterin | Homo sapiens | |
| 0.05 | - |
phenylalanine | recombinant enzyme, cofactor tetrahydrobiopterin | Homo sapiens | |
| 0.055 | - |
phenylalanine | recombinant enzyme, cofactor (7R)-5,6,7,8-tetrahydrobiopterin | Homo sapiens | |
| 0.2 | - |
(7R)-5,6,7,8-tetrahydrobiopterin | recombinant enzyme | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | incorporation of 0.6 mol phosphate per enzyme subunit, recombinant enzyme | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, Phenyl-Sepharose, DEAE-Sepharose, recombinant enzyme | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.6 | - |
recombinant enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-phenylalanine + (7R)-5,6,7,8-tetrahydrobiopterin + O2 | - |
Homo sapiens | L-tyrosine + 4a-hydroxytetrahydrobiopterin | - |
r | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0015 | - |
(7R)-5,6,7,8-tetrahydrobiopterin | - |
Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
