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Literature summary for 1.14.15.8 extracted from

  • Schmitz, D.; Zapp, J.; Bernhardt, R.
    Steroid conversion with CYP106A2 - production of pharmaceutically interesting DHEA metabolites (2014), Microb. Cell Fact., 13, 81 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.15.8

Application

Application Comment Organism
synthesis an overexpressing Bacillus megaterium strain produces up to 115 mg/l/h 7beta-hydroxydehydroepiandrosterone in whole-cell conversions Priestia megaterium

Cloned(Commentary)

Cloned (Comment) Organism
exression in Bacillus megaterium Priestia megaterium

Organism

Organism UniProt Comment Textmining
Priestia megaterium Q06069 isoform Cyp106A2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dehydroepiandrosterone + reduced adrenodoxin + O2
-
 Priestia megaterium 7beta-hydroxy-dehydroepiandrosterone + oxidized adrenodoxin + H2O
-
 ?
dexamethasone + reduced adrenodoxin + O2
-
 Priestia megaterium ? + oxidized adrenodoxin + H2O
-
 ?
digitoxigenin + reduced adrenodoxin + O2
-
 Priestia megaterium ? + oxidized adrenodoxin + H2O
-
 ?
additional information the 3-oxo-DELTA4 moiety is not crucial for steroid conversion with CYP106A2. No substrates: estrone, estradiol, stigmasterol, and cholesterol Priestia megaterium ?
-
 ?
prednisone + reduced adrenodoxin + O2
-
 Priestia megaterium ? + oxidized adrenodoxin + H2O
-
 ?
pregnenolone + reduced adrenodoxin + O2
-
 Priestia megaterium 7beta-hydroxy-pregnenolone + oxidized adrenodoxin + H2O
-
 ?