Cloned (Comment) | Organism |
---|---|
gene HvCMO, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative real-time PCR expression analyis, recombinant expression in Saccharomyces cerevisiae strain BJ5458 | Hordeum vulgare |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | no activity in mitochondria and the endoplasmic reticulum-containing microsome fraction, as well as chloroplasts | Hordeum vulgare | - |
- |
peroxisome | - |
Hordeum vulgare | 5777 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | in ferredoxin cofactor and a Rieske-type [2Fe-2S] cluster | Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44800 | - |
x * 44800, about, sequence calculation | Hordeum vulgare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
choline + O2 + 2 reduced ferredoxin + 2 H+ | Hordeum vulgare | - |
betaine aldehyde hydrate + H2O + 2 oxidized ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | B5UAE1 | cv. Haruna-nijyo | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
awn | - |
Hordeum vulgare | - |
floret | - |
Hordeum vulgare | - |
internode | - |
Hordeum vulgare | - |
leaf | high content of glycine betaine, more in the older leaf blades, but little in the florets and internodes | Hordeum vulgare | - |
seedling | - |
Hordeum vulgare | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
choline + O2 + 2 reduced ferredoxin + 2 H+ | - |
Hordeum vulgare | betaine aldehyde hydrate + H2O + 2 oxidized ferredoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44800, about, sequence calculation | Hordeum vulgare |
Synonyms | Comment | Organism |
---|---|---|
CMO | - |
Hordeum vulgare |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Hordeum vulgare |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Fe-S center | Rieske-type [2Fe-2S] cluster | Hordeum vulgare | |
Ferredoxin | dependent on | Hordeum vulgare |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Hordeum vulgare | sequence calculation | - |
6.2 |
Organism | Comment | Expression |
---|---|---|
Hordeum vulgare | the expression of HvCMO protein is increased by the presence of high NaCl, accumulation of HvCMO mRNA is increased by high osmotic and low-temperature environments | up |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the first stepin biosynthesis of glycine betaine. The second step is catalyzed by betaine aldehyde dehydrogenase | Hordeum vulgare |