Cloned (Comment) | Organism |
---|---|
gene OsCMO, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional OsCMO expression in transgenic Nicotiana tabacum cv. Xanthinc plants using Agrobacterium tumefaciens EHA105 for transfection, overexpressing plants show increased glycine betaine content and elevated tolerance to salt stress | Oryza sativa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | a mononuclear non-heme Fe2+, with binding sequence G/DX3-4DX2HX4-5H | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
* 43000, recombinant enzyme, SDS-PAGE | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | B8B239 | ssp. japonica, cv. Nipponbare, CMO single copy gene | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
choline + O2 + 2 reduced ferredoxin + 2 H+ | - |
Oryza sativa | betaine aldehyde hydrate + H2O + 2 oxidized ferredoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | * 43000, recombinant enzyme, SDS-PAGE | Oryza sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Fe-S center | Rieske-type [2Fe-2S] cluster sequence CXHX15-17CX2H | Oryza sativa |
General Information | Comment | Organism |
---|---|---|
metabolism | glycine betaine is a compatible quaternary amine that enables plants to tolerate abiotic stresses, including salt, drought and cold. In plants, glycine betaine is synthesized through two-step of successive oxidations from choline, catalyzed by choline monooxygenase and betaine aldehyde dehydrogenase, respectively. Oryza sativa is a typical non-glycine betaine accumulating species. The genome sequencing reveals orthologues of both choline monooxygenase and betaine aldehyde dehydrogenase, but, while the betaine aldehyde dehydrogenase is functional, the choline monooxygenase of japonica rice plant is not. Nevertheless the heterologously expressed rice enzyme is active in tabacco plants, overview | Oryza sativa |