Activating Compound | Comment | Organism | Structure |
---|---|---|---|
catalase | stimulation of activity by countering negative effects of H2O2 produced by ferredoxin autoxidation | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
betaine | 300 mM betaine give 50% inhibition | Spinacia oleracea | |
Zn2+ | 10 mM inhibits activity | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
choline | reduced ferredoxin provided by NADPH/ferredoxin-NADP+ reductase or reconstituted chloroplasts | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | in stromal fraction of chloroplasts | Spinacia oleracea | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM Ca2+ can replace Mg2+ | Spinacia oleracea | |
Mg2+ | maximum activity with more than 10 mM | Spinacia oleracea | |
additional information | Mn2+, Cu2+ or Co2+ have no effect on activity | Spinacia oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
SDS-PAGE | Spinacia oleracea |
98000 | - |
gel filtration with Ultrogel AcA 34 | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
spinach, cv. Savoy Hybrid 612 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00025 | - |
activity varies with leaf age | Spinacia oleracea |
0.00058 | - |
after salinization of plants with 200 mM NaCl for two weeks | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
choline + O2 + reduced ferredoxin | - |
Spinacia oleracea | betaine aldehyde hydrate + oxidized ferredoxin | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
reduced ferredoxin provided by NADPH/ferredoxin-NADP+ reductase or reconstituted chloroplasts | Spinacia oleracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | ferredoxin is reduced via NADPH and ferredoxin-NADP+ reductase | Spinacia oleracea | |
O2 | half-maximal rate with a O2 partial pressure of 2kPa, corresponding to an concentration of 0.025 mM | Spinacia oleracea |