Literature summary for 1.14.15.6 extracted from

Efimova, V.S.; Isaeva, L.V.; Makeeva, D.S.; Rubtsov, M.A.; Novikova, L.A.
Expression of cholesterol hydroxylase/lyase system proteins in yeast S. cerevisiae cells as a self-processing polyprotein (2017), Mol. Biotechnol., 59, 394-406 .

Search for more literature for 1.14.15.6

Application

Application	Comment	Organism
synthesis	construction of a fusion protein consisting of cytochrome P450scc (CYP11A1), adrenodoxin and adrenodoxin reductase including 2A peptide from Picornaviridae which is capable of self-cleavage. Introduction to Escherichia coli leads to a high level of expression but no cleavage. In yeast Saccharomyces cerevisiae, the discrete proteins P450scc-2A, adrenodoxin-2A and adrenodoxin reductase are expressed, with a significant proportion present in a fusion adrenodoxin-2A-adrenodoxin reductase. The enzyme system is catalytically active	Bos taurus

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli and Saccharomyces cerevisiae	Bos taurus

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a fusion protein consisting of cytochrome P450scc (CYP11A1), adrenodoxin and adrenodoxin reductase including 2A peptide from Picornaviridae which is capable of self-cleavage. Introduction to Escherichia coli leads to a high level of expression but no cleavage. In yeast Saccharomyces cerevisiae, the discrete proteins P450scc-2A, adrenodoxin-2A and adrenodoxin reductase are expressed, with a significant proportion present in a fusion adrenodoxin-2A-adrenodoxin reductase. The enzyme system is catalytically active	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	P00189	-	-

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)