Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of fusion proteins of enzyme plus adrenodoxin plus adrenodoxin reductase, fusion in order bovine enzyme - adrenodoxin - adrenodoxin reductase gives 30-fold higher enzymatic activity than fusion in same order with human enzyme and 14-fold higher activity than fusion with human enzyme in order adrenodoxin reductase adrenodoxin enzyme. Dimers of enzyme with adrenodoxin in order enzyme adrenodoxin or adrenodoxin enzyme show minimal side chain cleavage activity. CO difference spectra reveal a denatured cytochrome P450 in dimer fusion proteins | Homo sapiens |
additional information | construction of fusion proteins of enzyme plus adrenodoxin plus adrenodoxin reductase, fusion in order enzyme - adrenodoxin - adrenodoxin reductase gives 30-fold higher enzymatic activity than fusion in same order with human enzyme and 14-fold higher activity than fusion with human enzyme in order adrenodoxin reductase adrenodoxin enzyme. CO-difference spectra do not show the presence of a normally folded enzyme moiety | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
x * 120000, fusion protein of enzyme plus adrenodoxin reductase plus adrenodoxin | Homo sapiens |
120000 | - |
x * 120000, fusion protein of enzyme plus adrenodoxin reductase plus adrenodoxin | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
fusion protein with adrenodoxin and/or adrenodoxin reductase | - |
Homo sapiens | - |
fusion protein with adrenodoxin and/or adrenodoxin reductase | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
22(R)-hydroxycholesterol + reduced adrenodoxin + O2 | - |
Homo sapiens | pregnenolone + oxidized adrenodoxin + H2O | - |
? | |
22(R)-hydroxycholesterol + reduced adrenodoxin + O2 | - |
Bos taurus | pregnenolone + oxidized adrenodoxin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 120000, fusion protein of enzyme plus adrenodoxin reductase plus adrenodoxin | Homo sapiens |
? | x * 120000, fusion protein of enzyme plus adrenodoxin reductase plus adrenodoxin | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Homo sapiens | |
cytochrome P450 | - |
Bos taurus |