Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1,2-di-(2'-hexyl-decanoyl)-sn-glycero-3-phosphocholine | alpha-branched phosphatidylcholine, inclusion in vesicle-reconstituted system, partially in connection with the nonactivator lipids dimyristoyl-/dioleoyl-phosphatidylcholine, efficiency close to cardiolipin | Bos taurus | |
1,2-di-(2'-octyl-dodecanoyl)-sn-glycero-phosphocholine | alpha-branched phosphatidylcholine, inclusion in vesicle-reconstituted system, partially in connection with the nonactivator lipids dimyristoyl-/dioleoyl-phosphatidylcholine, efficiency close to cardiolipin | Bos taurus | |
cardiolipin | most potent and effective activator lipid, binds to enzyme and enhances the binding of cholesterol | Bos taurus | |
additional information | head and fatty acid groups have large effects on stimulation of activity, head group appears as major determinant of the lipid interaction with the P-450scc, 1-2 and 3-4 molecules of cardiolipin and the branched phosphatidylcholines, respectively, bind highly specific to P-450scc at an effector site distant from cholesterol binding site with a resultant stabilization of an optimal cholesterol-binding conformation of P-450scc | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | inner membrane | Bos taurus | 5743 | - |
mitochondrial inner membrane | matrix side | Bos taurus | 5743 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + 6 reduced adrenodoxin + 3 O2 | Bos taurus | - |
pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O | precursor of all steroid hormones | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + 6 reduced adrenodoxin + 3 O2 | - |
Bos taurus | pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O | precursor of all steroid hormones | ? | |
cholesterol + reduced adrenodoxin + O2 | - |
Bos taurus | pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 42 | tested in different dimyristoyl-phosphatidylcholine vesicles, breaks in activity at 27-30°C | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
adrenodoxin | - |
Bos taurus | |
heme | - |
Bos taurus | |
NADPH | NADPH-linked adrenodoxin reductase | Bos taurus |