Literature summary for 1.14.15.4 extracted from

Sato, H.; Ashida, N.; Suhara, K.; Itagaki, E.; Takemori, S.; Katagiri, M.
Properties of an adrenal cytochrome P-450 (P-45011beta) for the hydroxylations of corticosteroids (1978), Arch. Biochem. Biophys., 190, 307-314.

Search for more literature for 1.14.15.4

Inhibitors

Inhibitors	Comment	Organism	Structure
CO	affects 11beta-/19-hydroxylase reaction	Bos taurus
Metyrapone	competitive with substrate, affects strongly 11beta-/18-hydroxylation and 11beta-/19-hydroxylation	Bos taurus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.006	-	deoxycorticosterone	identical for both enzyme activities	Bos taurus
0.013	-	4-androstene-3,17-dione	identical for both enzyme activities	Bos taurus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Bos taurus	5739	-

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
copurification of 11beta-/18-hydroxylase with 11beta-/19-hydroxylase, cholate extraction	Bos taurus

Reaction

Reaction	Comment	Organism	Reaction ID
a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O	P-45011beta responsible for two hydroxylase reactions	Bos taurus	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
adrenal cortex	-	Bos taurus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	activities of sonicated mitochondria and cholate extract of hydroxylation activities with deoxycotricosterone and 4-androstene-3,17-dione as substrates	Bos taurus

Storage Stability

Storage Stability	Organism
-80°C, 59 mM potassium phosphate buffer, pH 7.4, 0.1 mM EDTA, 0.1 mM dithiothreitol, 0.01 mM deoxycorticosterone, 0.3% cholate, 0.3% Tween 20	Bos taurus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
11-deoxycorticosterone + reduced adrenal ferredoxin + O2	containing adrenal ferredoxin, adrenal ferredoxin reductase and P-45011beta, highly active hydroxylation, 11beta-/18-hydroxylase activity	Bos taurus	corticosterone + oxidized adrenal ferredoxin + H2O	-	?
4-androstene-3,17-dione + reduced adrenal ferredoxin + O2	i.e. testosterone, 11beta-/19-hydroxylase activity, good sustrate	Bos taurus	11beta-hydroxy-4-androstene-3,17-dione + oxidized adrenal ferredoxin + H2O	-	?
4-androstene-3,17-dione + reduced adrenal ferredoxin + O2	i.e. testosterone, 11beta-/19-hydroxylase activity, good sustrate	Bos taurus	19-hydroxy-4-androstene-3,17-dione + oxidized adrenal ferredoxin + H2O	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.3	-	11-deoxycorticosterone	18-hydroxylation	Bos taurus
1.83	-	11-deoxycorticosterone	11beta-hydroxylation	Bos taurus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Bos taurus

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Bos taurus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)