Application | Comment | Organism |
---|---|---|
synthesis | construction of an artificial self-sufficient cytochrome P450 monooxygenase by fusion of NADPH-P450 reductase FprD, CYP51, and iron-sulfur containing FprD. CYP51-FprD fusion enzymes F1 and F2 in recombinant Escherichia coli catalyze demethylation of lanosterol more efficiently, with kcat/Km values about 35fold higher compared to those of CYP51 and FprD alone | Nocardia farcinica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0103 | - |
lanosterol | FprD fusion construct F1, pH 7.0, temperature not specified in the publication | Nocardia farcinica | |
0.0122 | - |
lanosterol | wild-type, pH 7.0, temperature not specified in the publication | Nocardia farcinica | |
0.0125 | - |
lanosterol | FprD fusion construct F2, pH 7.0, temperature not specified in the publication | Nocardia farcinica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocardia farcinica | Q5YWK5 | - |
- |
Nocardia farcinica IFM 10152 | Q5YWK5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2 | - |
Nocardia farcinica | 4,4-dimethylzymosterol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O | - |
? | |
lanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2 | - |
Nocardia farcinica IFM 10152 | 4,4-dimethylzymosterol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.67 | - |
lanosterol | wild-type, pH 7.0, temperature not specified in the publication | Nocardia farcinica | |
16.7 | - |
lanosterol | FprD fusion construct F1, pH 7.0, temperature not specified in the publication | Nocardia farcinica | |
21.9 | - |
lanosterol | FprD fusion construct F2, pH 7.0, temperature not specified in the publication | Nocardia farcinica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
50.5 | - |
lanosterol | wild-type, pH 7.0, temperature not specified in the publication | Nocardia farcinica | |
1615 | - |
lanosterol | FprD fusion construct F1, pH 7.0, temperature not specified in the publication | Nocardia farcinica | |
1763 | - |
lanosterol | FprD fusion construct F2, pH 7.0, temperature not specified in the publication | Nocardia farcinica |