Literature summary for 1.14.15.36 extracted from

Choi, K.Y.; Jung, E.O.; Jung, D.H.; Pandey, B.P.; Lee, N.; Yun, H.; Park, H.Y.; Kim, B.G.
Novel iron-sulfur containing NADPH-reductase from Nocardia farcinica IFM10152 and fusion construction with CYP51 lanosterol demethylase (2012), Biotechnol. Bioeng., 109, 630-636 .

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Application

Application	Comment	Organism
synthesis	construction of an artificial self-sufficient cytochrome P450 monooxygenase by fusion of NADPH-P450 reductase FprD, CYP51, and iron-sulfur containing FprD. CYP51-FprD fusion enzymes F1 and F2 in recombinant Escherichia coli catalyze demethylation of lanosterol more efficiently, with kcat/Km values about 35fold higher compared to those of CYP51 and FprD alone	Nocardia farcinica

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0103	-	lanosterol	FprD fusion construct F1, pH 7.0, temperature not specified in the publication	Nocardia farcinica
0.0122	-	lanosterol	wild-type, pH 7.0, temperature not specified in the publication	Nocardia farcinica
0.0125	-	lanosterol	FprD fusion construct F2, pH 7.0, temperature not specified in the publication	Nocardia farcinica

Organism

Organism	UniProt	Comment	Textmining
Nocardia farcinica	Q5YWK5	-	-
Nocardia farcinica IFM 10152	Q5YWK5	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
lanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2	-	Nocardia farcinica	4,4-dimethylzymosterol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O	-	?
lanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2	-	Nocardia farcinica IFM 10152	4,4-dimethylzymosterol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.67	-	lanosterol	wild-type, pH 7.0, temperature not specified in the publication	Nocardia farcinica
16.7	-	lanosterol	FprD fusion construct F1, pH 7.0, temperature not specified in the publication	Nocardia farcinica
21.9	-	lanosterol	FprD fusion construct F2, pH 7.0, temperature not specified in the publication	Nocardia farcinica

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
50.5	-	lanosterol	wild-type, pH 7.0, temperature not specified in the publication	Nocardia farcinica
1615	-	lanosterol	FprD fusion construct F1, pH 7.0, temperature not specified in the publication	Nocardia farcinica
1763	-	lanosterol	FprD fusion construct F2, pH 7.0, temperature not specified in the publication	Nocardia farcinica

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Release 2023.1 (January 2023)