Cloned (Comment) | Organism |
---|---|
gene eryF, genetic structure, sequence comparison, recombinant expression of His6-tagged enzyme in Saccharopolyspora erythraea strain ZL2001 | Saccharopolyspora erythraea |
gene eryF, whole-genome sequencing, DNA and amino acid sequence determination and analysis, genetic structure,sequence comparison, genomic DNA of Saccharopolyspora erythraea strain ZL2001 serves as template for PCR amplification, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), recombinant expression in and complementation fo Saccharopolyspora erythraea eryF-deletion mutant strain EX105(ZL2001) | Actinopolyspora erythraea |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an erythronolide H-producing Saccharopolyspora erythraea mutant via gene complementation is not successful. Construction of en eryF deletion mutant strainEX105 from strain ZL2001 and complementation by Actinopolyspora erythraea eryF expression | Saccharopolyspora erythraea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | Saccharopolyspora erythraea | C-6 hydroxylation | erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | Actinopolyspora erythraea | C-6 hydroxylation | erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | Actinopolyspora erythraea | a two-step oxidation (14-hydroxylation and 6,18-epoxyidation) via the intermediate erythronolide B | erythronolide H + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | Saccharopolyspora erythraea NRRL2338 | C-6 hydroxylation | erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | Actinopolyspora erythraea YIM90600 | C-6 hydroxylation | erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | Actinopolyspora erythraea YIM90600 | a two-step oxidation (14-hydroxylation and 6,18-epoxyidation) via the intermediate erythronolide B | erythronolide H + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
additional information | Actinopolyspora erythraea | the enzyme plays a role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide in vitro | ? | - |
? | |
additional information | Actinopolyspora erythraea YIM90600 | the enzyme plays a role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide in vitro | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Actinopolyspora erythraea | A0A099D4T8 | halophilic actinomycete isolated from Baicheng salt field | - |
Actinopolyspora erythraea YIM90600 | A0A099D4T8 | halophilic actinomycete isolated from Baicheng salt field | - |
Saccharopolyspora erythraea | Q00441 | - |
- |
Saccharopolyspora erythraea NRRL2338 | Q00441 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | C-6 hydroxylation | Saccharopolyspora erythraea | erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | C-6 hydroxylation | Actinopolyspora erythraea | erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | C-6 hydroxylation | Saccharopolyspora erythraea NRRL2338 | erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | C-6 hydroxylation | Actinopolyspora erythraea YIM90600 | erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | a two-step oxidation (14-hydroxylation and 6,18-epoxyidation) via the intermediate erythronolide B | Actinopolyspora erythraea | erythronolide H + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | a two-step oxidation (14-hydroxylation and 6,18-epoxyidation) via the intermediate erythronolide B | Actinopolyspora erythraea YIM90600 | erythronolide H + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
additional information | the enzyme plays a role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide in vitro | Actinopolyspora erythraea | ? | - |
? | |
additional information | the enzyme plays a role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide in vitro | Actinopolyspora erythraea YIM90600 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
eryF | - |
Saccharopolyspora erythraea |
eryF | - |
Actinopolyspora erythraea |
EryFAc | - |
Actinopolyspora erythraea |
EryFSa | - |
Saccharopolyspora erythraea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharopolyspora erythraea |
30 | - |
assay at | Actinopolyspora erythraea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharopolyspora erythraea |
7.5 | - |
assay at | Actinopolyspora erythraea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Saccharopolyspora erythraea | |
cytochrome P450 | - |
Actinopolyspora erythraea | |
Ferredoxin | - |
Saccharopolyspora erythraea | |
Ferredoxin | - |
Actinopolyspora erythraea | |
ferredoxin [iron-sulfur] cluster | - |
Saccharopolyspora erythraea | |
ferredoxin [iron-sulfur] cluster | - |
Actinopolyspora erythraea |
General Information | Comment | Organism |
---|---|---|
evolution | the Er gene cluster from Actinopolyspora erythraea strain YIM90600 shares high identity and similarity with the one of Saccharopolyspora erythraea strain NRRL2338, except for two absent genes, eryBI and eryG, correlation of genotype and chemotype | Saccharopolyspora erythraea |
evolution | the Er gene cluster from Actinopolyspora erythraea strain YIM90600 shares high identity and similarity with the one of Saccharopolyspora erythraea strain NRRL2338, except for two absent genes, eryBI and eryG. By correlating genotype and chemotype, the biosynthetic pathways of 3'-demethyl-erythromycin C, erythronolide H (EH) and erythronolide I are proposed. Formation of erythronolide H is supposed to be sequentially biosynthesized via C-6/C-18 epoxidation and C-14 hydroxylation from 6-deoxyerythronolide B | Actinopolyspora erythraea |
metabolism | the enzyme is involved in the biosynthetic pathway of the final erythronolide product, erythronolid A, pathway overview | Saccharopolyspora erythraea |
metabolism | the enzyme is involved in the biosynthetic pathway of the final erythronolide product, erythronolid A, pathway overview | Actinopolyspora erythraea |