Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion crystallization, crystal structures of the ferrous dioxygen complex of wild-type enzyme and its mutants, A245S and A245T | Saccharopolyspora erythraea |
Protein Variants | Comment | Organism |
---|---|---|
A245S | activity is decreased by 84%, the active site structure including water is essentially unchanged with the exception that the OH group of Ser245 points toward the I-helix cleft to make new H-bonds with water 63 and the carbonyl group of the Ala241 | Saccharopolyspora erythraea |
A245T | more than 99% loss of activity | Saccharopolyspora erythraea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | Saccharopolyspora erythraea | the enzyme is involved in the biosynthesis of the antibiotic erythromycin | erythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | Saccharopolyspora erythraea NRRL 2338 | the enzyme is involved in the biosynthesis of the antibiotic erythromycin | erythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharopolyspora erythraea | Q00441 | - |
- |
Saccharopolyspora erythraea NRRL 2338 | Q00441 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharopolyspora erythraea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | the enzyme is involved in the biosynthesis of the antibiotic erythromycin | Saccharopolyspora erythraea | erythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | direct involvement of the substrate in O2 activation | Saccharopolyspora erythraea | erythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | the enzyme is involved in the biosynthesis of the antibiotic erythromycin | Saccharopolyspora erythraea NRRL 2338 | erythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | direct involvement of the substrate in O2 activation | Saccharopolyspora erythraea NRRL 2338 | erythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP107A1 | - |
Saccharopolyspora erythraea |
DEB hydroxylase | - |
Saccharopolyspora erythraea |
eryF | - |
Saccharopolyspora erythraea |
P450eryF | - |
Saccharopolyspora erythraea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | a heme-thiolate protein (P-450) | Saccharopolyspora erythraea |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of the antibiotic erythromycin | Saccharopolyspora erythraea |