Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces venezuelae |
Crystallization (Comment) | Organism |
---|---|
- |
Streptomyces venezuelae |
Protein Variants | Comment | Organism |
---|---|---|
D50N | the mutant enzyme provides more efficient hydroxylation of both macrolide substrates when compared with native enzyme | Streptomyces venezuelae |
E85A/E94A | the mutant enzyme is catalytically incompetent toward both endogenous macrolactone substrates | Streptomyces venezuelae |
E85Q | change in product profile, mutation shifts the product ratio of 10-deoxymethymycin hydroxylation toward methymycin | Streptomyces venezuelae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | Streptomyces venezuelae | involved in the biosynthesis of a number of bacterial macrolide antibiotics containing a desosamine glycoside unit | novamethymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | Streptomyces venezuelae | involved in the biosynthesis of a number of bacterial macrolide antibiotics containing a desosamine glycoside unit | methymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | Streptomyces venezuelae | involved in the biosynthesis of a number of bacterial macrolide antibiotics containing a desosamine glycoside unit | neomethymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | Streptomyces venezuelae | involved in the biosynthesis of a number of bacterial macrolide antibiotics containing a desosamine glycoside unit | novapikromyin + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? | |
narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | Streptomyces venezuelae | involved in the biosynthesis of a number of bacterial macrolide antibiotics containing a desosamine glycoside unit | neopikromycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | Streptomyces venezuelae | involved in the biosynthesis of a number of bacterial macrolide antibiotics containing a desosamine glycoside unit | pikromycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces venezuelae | O87605 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces venezuelae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = methymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | (4) | Streptomyces venezuelae | |
10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = neomethymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | (5) | Streptomyces venezuelae | |
10-deoxymethymycin + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H+ + 2 O2 = novamethymycin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | (6) | Streptomyces venezuelae | |
narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = neopikromycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | (2) | Streptomyces venezuelae | |
narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = pikromycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | (1) | Streptomyces venezuelae | |
narbomycin + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H+ + 2 O2 = novapikromyin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | (3) | Streptomyces venezuelae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | involved in the biosynthesis of a number of bacterial macrolide antibiotics containing a desosamine glycoside unit | Streptomyces venezuelae | novamethymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | with 10-deoxymethymycin it hydroxylates at either C-10 to give methymycin or C-12 to give neomethymycin or both positions to give novamethymycin | Streptomyces venezuelae | novamethymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | involved in the biosynthesis of a number of bacterial macrolide antibiotics containing a desosamine glycoside unit | Streptomyces venezuelae | methymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | with 10-deoxymethymycin it hydroxylates at either C-10 to give methymycin or C-12 to give neomethymycin or both positions to give novamethymycin | Streptomyces venezuelae | methymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | involved in the biosynthesis of a number of bacterial macrolide antibiotics containing a desosamine glycoside unit | Streptomyces venezuelae | neomethymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | with 10-deoxymethymycin it hydroxylates at either C-10 to give methymycin or C-12 to give neomethymycin or both positions to give novamethymycin | Streptomyces venezuelae | neomethymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | involved in the biosynthesis of a number of bacterial macrolide antibiotics containing a desosamine glycoside unit | Streptomyces venezuelae | novapikromyin + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? | |
narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | with narbomycin the enzyme hydroxylates at either C-12 to give pikromycin or C-14 to give neopikromycin or both positions to give narvopikromycin | Streptomyces venezuelae | novapikromyin + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? | |
narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | involved in the biosynthesis of a number of bacterial macrolide antibiotics containing a desosamine glycoside unit | Streptomyces venezuelae | neopikromycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | with narbomycin the enzyme hydroxylates at either C-12 to give pikromycin or C-14 to give neopikromycin or both positions to give narvopikromycin | Streptomyces venezuelae | neopikromycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | involved in the biosynthesis of a number of bacterial macrolide antibiotics containing a desosamine glycoside unit | Streptomyces venezuelae | pikromycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | with narbomycin the enzyme hydroxylates at either C-12 to give pikromycin or C-14 to give neopikromycin or both positions to give narvopikromycin | Streptomyces venezuelae | pikromycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP107L1 | - |
Streptomyces venezuelae |
PikC | - |
Streptomyces venezuelae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces venezuelae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
assay at | Streptomyces venezuelae |