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Literature summary for 1.14.15.33 extracted from

  • Lee, S.K.; Basnet, D.B.; Choi, C.Y.; Sohng, J.K.; Ahn, J.S.; Yoon, Y.J.
    The role of erythromycin C-12 hydroxylase, EryK, as a substitute for PikC hydroxylase in pikromycin biosynthesis (2004), Bioorg. Chem., 32, 549-559.
    View publication on PubMed
Search for more literature for 1.14.15.33

Cloned(Commentary)

Cloned (Comment) Organism
expression in a Streptomyces venezuelae pikC mutant Saccharopolyspora erythraea

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular modeling of the active site. For substrate narbomycin, the hydrophobic residues Ala223, Leu224, Leu225, and Leu226 may form hydrophobic interactions with the methyl group at C-6 and C-8 including C-7 of the macrolide ring Saccharopolyspora erythraea

Organism

Organism UniProt Comment Textmining
Saccharopolyspora erythraea P48635 erythromycin C-12 hydroxylase is able to substitute for pikromycin hydroxylase which is responsible for the hydroxylation of 10-deoxymethymycin and narbomycin. Erythromycin C-12 hydroxylase accepts both 12- and 14-membered macrolides
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
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 Saccharopolyspora erythraea methymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Saccharopolyspora erythraea neomethymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
additional information erythromycin C-12 hydroxylase is able to substitute for pikromycin hydroxylase which is responsible for the hydroxylation of 10-deoxymethymycin and narbomycin. Erythromycin C-12 hydroxylase accepts both 12- and 14-membered macrolides Saccharopolyspora erythraea ?
-
 ?
narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Saccharopolyspora erythraea pikromycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

Synonyms Comment Organism
erythromycin C-12 hydroxylase
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 Saccharopolyspora erythraea