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Literature summary for 1.14.15.30 extracted from

  • Van Der Geize, R.; Hessels, G.; Van Gerwen, R.; Van Der Meijden, P.; Dijkhuizen, L.
    Molecular and functional characterization of kshA and kshB, encoding two components of 3-ketosteroid 9alpha-hydroxylase, a class IA monooxygenase, in Rhodococcus erythropolis strain SQ1 (2002), Mol. Microbiol., 45, 1007-1018.
    View publication on PubMed
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Protein Variants

Protein Variants Comment Organism
additional information unmarked in frame gene deletion mutant of parent strain Rhodococcus erythropolis strain SQ1, designated strain RG2 (kshA mutant), is unable to grow on steroid substrates androsta-1,4-diene-3,17-dione and 4-androstene-3,17-dione, whereas growth on 9alpha-hydroxy-4-androstene-3,17-dione is not affected Rhodococcus erythropolis
additional information unmarked in frame gene deletion mutant of parent strain Rhodococcus erythropolis strain SQ1, designated strain RG4 (kshB mutant), is unable to grow on steroid substrates androsta-1,4-diene-3,17-dione and 4-androstene-3,17-dione, whereas growth on 9alpha-hydroxy-4-androstene-3,17-dione is not affected. Strain RG4 is also impaired in sterol degradation Rhodococcus erythropolis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ contains non-heme iron Rhodococcus erythropolis

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis Q8KQH9 component KshB of 3-ketosteroid 9alpha-hydroxylase
-
Rhodococcus erythropolis Q8KQI0 component KshA of 3-ketosteroid 9alpha-hydroxylase
-
Rhodococcus erythropolis SQ1 Q8KQH9 component KshB of 3-ketosteroid 9alpha-hydroxylase
-
Rhodococcus erythropolis SQ1 Q8KQI0 component KshA of 3-ketosteroid 9alpha-hydroxylase
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-androstene-3,17-dione + O2 + NADH + H+
-
 Rhodococcus erythropolis 9alpha-hydroxy-4-androstene-3,17-dione + H2O + NAD+
-
 ?
4-androstene-3,17-dione + O2 + NADH + H+
-
 Rhodococcus erythropolis SQ1 9alpha-hydroxy-4-androstene-3,17-dione + H2O + NAD+
-
 ?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Rhodococcus erythropolis 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Rhodococcus erythropolis SQ1 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

Synonyms Comment Organism
3-ketosteroid 9alpha-hydroxylase
-
 Rhodococcus erythropolis
KSH
-
 Rhodococcus erythropolis
KshA terminal oxygenase component of 3-ketosteroid 9alpha-hydroxylase, kshA encodes both androsta-1,4-diene-3,17-dione and 4-androstene-3,17-dione 9alpha-hydroxylase activity Rhodococcus erythropolis
KshB the KshB protein can be assigned to the class IA oxygenase ferredoxin reductases and represents the ferredoxin reductase component of 3-ketosteroid 9alpha-hydroxylase Rhodococcus erythropolis

Cofactor

Cofactor Comment Organism Structure
[2Fe-2S]-center
-
 Rhodococcus erythropolis