BRENDA - Enzyme Database
show all sequences of 1.14.15.30

Molecular and functional characterization of kshA and kshB, encoding two components of 3-ketosteroid 9alpha-hydroxylase, a class IA monooxygenase, in Rhodococcus erythropolis strain SQ1

Van Der Geize, R.; Hessels, G.; Van Gerwen, R.; Van Der Meijden, P.; Dijkhuizen, L.; Mol. Microbiol. 45, 1007-1018 (2002)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
additional information
unmarked in frame gene deletion mutant of parent strain Rhodococcus erythropolis strain SQ1, designated strain RG2 (kshA mutant), is unable to grow on steroid substrates androsta-1,4-diene-3,17-dione and 4-androstene-3,17-dione, whereas growth on 9alpha-hydroxy-4-androstene-3,17-dione is not affected; unmarked in frame gene deletion mutant of parent strain Rhodococcus erythropolis strain SQ1, designated strain RG4 (kshB mutant), is unable to grow on steroid substrates androsta-1,4-diene-3,17-dione and 4-androstene-3,17-dione, whereas growth on 9alpha-hydroxy-4-androstene-3,17-dione is not affected. Strain RG4 is also impaired in sterol degradation
Rhodococcus erythropolis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
contains non-heme iron; contains non-heme iron
Rhodococcus erythropolis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodococcus erythropolis
Q8KQH9
component KshB of 3-ketosteroid 9alpha-hydroxylase
-
Rhodococcus erythropolis
Q8KQI0
component KshA of 3-ketosteroid 9alpha-hydroxylase
-
Rhodococcus erythropolis SQ1
Q8KQH9
component KshB of 3-ketosteroid 9alpha-hydroxylase
-
Rhodococcus erythropolis SQ1
Q8KQI0
component KshA of 3-ketosteroid 9alpha-hydroxylase
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-androstene-3,17-dione + O2 + NADH + H+
-
718125
Rhodococcus erythropolis
9alpha-hydroxy-4-androstene-3,17-dione + H2O + NAD+
-
-
-
?
4-androstene-3,17-dione + O2 + NADH + H+
-
718125
Rhodococcus erythropolis SQ1
9alpha-hydroxy-4-androstene-3,17-dione + H2O + NAD+
-
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
718125
Rhodococcus erythropolis
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
718125
Rhodococcus erythropolis SQ1
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
[2Fe-2S]-center
;
Rhodococcus erythropolis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
[2Fe-2S]-center
-
Rhodococcus erythropolis
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
unmarked in frame gene deletion mutant of parent strain Rhodococcus erythropolis strain SQ1, designated strain RG2 (kshA mutant), is unable to grow on steroid substrates androsta-1,4-diene-3,17-dione and 4-androstene-3,17-dione, whereas growth on 9alpha-hydroxy-4-androstene-3,17-dione is not affected
Rhodococcus erythropolis
additional information
unmarked in frame gene deletion mutant of parent strain Rhodococcus erythropolis strain SQ1, designated strain RG4 (kshB mutant), is unable to grow on steroid substrates androsta-1,4-diene-3,17-dione and 4-androstene-3,17-dione, whereas growth on 9alpha-hydroxy-4-androstene-3,17-dione is not affected. Strain RG4 is also impaired in sterol degradation
Rhodococcus erythropolis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
contains non-heme iron
Rhodococcus erythropolis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-androstene-3,17-dione + O2 + NADH + H+
-
718125
Rhodococcus erythropolis
9alpha-hydroxy-4-androstene-3,17-dione + H2O + NAD+
-
-
-
?
4-androstene-3,17-dione + O2 + NADH + H+
-
718125
Rhodococcus erythropolis SQ1
9alpha-hydroxy-4-androstene-3,17-dione + H2O + NAD+
-
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
718125
Rhodococcus erythropolis
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
718125
Rhodococcus erythropolis SQ1
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Other publictions for EC 1.14.15.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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22
1
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1
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22
1
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4
4
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-
-
746503
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5
1207
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1
-
1
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5
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8
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2
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1
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1
10
2
1
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1
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5
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1
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1
1
10
2
1
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1
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1
1
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743221
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1
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1
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3
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1
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3
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1
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1
-
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1
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1
-
-
744058
Petrusma
3-Ketosteroid 9alpha-hydroxyl ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Mycolicibacterium smegmatis, Mycolicibacterium smegmatis mc2 155, Rhodococcus erythropolis, Rhodococcus erythropolis SQ1, Rhodococcus jostii, Rhodococcus rhodochrous, Rhodococcus rhodochrous DSM 43269
Antonie van Leeuwenhoek
106
157-172
2014
-
2
2
-
1
-
-
-
-
5
-
22
-
15
-
-
-
5
-
-
-
-
28
5
-
-
-
-
-
-
-
10
-
-
-
-
2
4
14
-
1
-
-
-
-
-
-
7
-
22
-
-
-
-
-
-
-
-
28
7
-
-
-
-
-
-
-
-
-
20
28
-
-
-
745308
Penfield
Substrate specificities and c ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Rhodococcus rhodochrous, Rhodococcus rhodochrous DSM43269, Rhodococcus rhodochrous DSM 43269
J. Biol. Chem.
289
25523-25536
2014
-
-
1
2
-
-
4
19
-
-
-
8
-
18
-
-
1
2
-
-
-
-
54
2
2
-
-
19
2
-
-
-
3
-
-
-
-
2
-
3
-
-
-
6
3
20
-
-
-
8
-
-
-
2
-
-
-
-
54
3
3
-
-
19
3
-
-
-
-
4
6
-
17
17
745698
Yeh
Deletion of the gene encoding ...
Rhodococcus hoagii 103S, Rhodococcus hoagii
Microb. Cell Fact.
13
130
2014
-
-
1
-
1
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
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1
-
-
1
-
-
-
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-
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4
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
717737
Petrusma
Structural features in the Ksh ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous DSM 43269
J. Bacteriol.
194
115-121
2012
-
-
1
-
8
-
-
2
-
1
-
3
-
13
-
-
1
-
-
-
-
-
74
-
-
-
-
-
1
-
-
2
-
-
-
-
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3
4
-
16
-
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-
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4
-
2
-
3
-
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-
3
-
-
-
-
74
-
-
-
-
-
2
-
-
-
-
1
2
-
-
-
717735
Petrusma
Multiplicity of 3-ketosteroid- ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous DSM 43269
J. Bacteriol.
193
3931-3940
2011
-
-
1
-
1
-
-
-
-
-
-
4
-
19
-
-
1
-
-
1
-
-
75
-
-
-
-
-
1
-
-
1
-
-
-
-
-
6
5
-
5
-
-
-
-
-
-
-
-
4
-
-
-
6
-
3
-
-
75
-
-
-
-
-
3
-
-
-
1
3
11
5
-
-
717854
Capyk
Activity of 3-ketosteroid 9alp ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Biol. Chem.
286
40717-40724
2011
-
-
-
-
-
-
-
13
-
-
-
2
-
9
-
-
-
-
-
-
-
-
30
1
1
-
-
11
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
21
-
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-
2
-
-
-
-
-
-
-
-
30
2
2
-
-
19
2
-
-
-
-
2
3
-
13
19
713784
Wei
A new steroid-transforming str ...
Mycolicibacterium neoaurum, Mycolicibacterium neoaurum NwIB-01
Appl. Biochem. Biotechnol.
162
1446-1456
2010
-
-
-
-
-
-
-
-
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1
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3
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2
1
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1
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1
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2
1
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-
-
-
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1
-
1
1
-
-
-
716250
Hu
3-Ketosteroid 9alpha-hydroxyla ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Mol. Microbiol.
75
107-121
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
160
-
-
-
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4
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4
-
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-
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-
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-
-
2
2
-
-
-
717500
Fan
Cloning, heterologous expressi ...
Mycobacterium sp., Mycobacterium sp. NwIB-01
Chin. J. Biotechnol.
25
2014-2021
2009
-
-
1
-
-
-
-
-
-
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1
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8
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1
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1
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1
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1
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1
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1
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-
-
-
-
-
-
-
-
-
-
717784
Capyk
Characterization of 3-ketoster ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Biol. Chem.
284
9937-9946
2009
-
-
1
1
-
-
-
3
-
-
-
-
-
6
-
-
1
-
-
-
-
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4
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3
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1
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1
1
1
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3
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1
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4
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3
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-
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-
-
3
3
717119
Van Der Geize
Characterization of a second R ...
Rhodococcus erythropolis, Rhodococcus erythropolis SQ1
Appl. Environ. Microbiol.
74
7197-7203
2008
-
-
-
-
-
-
-
-
-
-
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9
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8
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1
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8
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1
2
3
1
-
-
717118
Andor
Generation of useful insertion ...
Mycolicibacterium smegmatis, Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
Appl. Environ. Microbiol.
72
6554-6559
2006
-
-
1
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1
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718125
Van Der Geize
Molecular and functional chara ...
Rhodococcus erythropolis, Rhodococcus erythropolis SQ1
Mol. Microbiol.
45
1007-1018
2002
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1
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1
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2
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8
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