Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | B2ZFP6 | terminal oxygenase homologue KshA2 | - |
Rhodococcus erythropolis | B5TYS8 | KshA3 | - |
Rhodococcus erythropolis SQ1 | B2ZFP6 | terminal oxygenase homologue KshA2 | - |
Rhodococcus erythropolis SQ1 | B5TYS8 | KshA3 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-androstene-3,17-dione + O2 + NADH + H+ | - |
Rhodococcus erythropolis | 9alpha-hydroxyandrosta-4-en-3,17-dione + H2O + NAD+ | - |
ir | |
4-androstene-3,17-dione + O2 + NADH + H+ | - |
Rhodococcus erythropolis SQ1 | 9alpha-hydroxyandrosta-4-en-3,17-dione + H2O + NAD+ | - |
ir | |
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Rhodococcus erythropolis | 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
ir | |
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Rhodococcus erythropolis SQ1 | 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
3-ketosteroid 9alpha-hydroxylase | - |
Rhodococcus erythropolis |
KSH | - |
Rhodococcus erythropolis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
[2Fe-2S]-center | - |
Rhodococcus erythropolis |
Organism | Comment | Expression |
---|---|---|
Rhodococcus erythropolis | expression of kshA2 is induced in parent strain Rhodococcus erythropolis SQ1 in the presence of androsta-1,4-diene-3,17-dione or 9alpha-hydroxy-4-androstene-3,17-dione or a metabolite thereof | up |
General Information | Comment | Organism |
---|---|---|
malfunction | gene deletion of kshA2 does not affect growth on 9alpha-hydroxy-4-androstene-3,17-dione, cholesterol, or cholic acid | Rhodococcus erythropolis |
physiological function | 3-ketosteroid 9alpha-hydroxylase is a key enzyme in microbial steroid degradation in Rhodococcus erythropolis strain SQ1 and is comprised of the terminal oxygenase component KshA1 and the oxygenase-reductase component KshB, a terminal oxygenase homologue KshA2 and homologue KshA3. Component KshA2 plays a role in preventing accumulation of toxic intracellular concentrations of 1,4-androstadiene-3,17-dione during steroid catabolism | Rhodococcus erythropolis |