BRENDA - Enzyme Database
show all sequences of 1.14.15.30

Characterization of a second Rhodococcus erythropolis SQ1 3-ketosteroid 9alpha-hydroxylase activity comprising a terminal oxygenase homologue, KshA2, active with oxygenase-reductase component KshB

Van Der Geize, R.; Hessels, G.; Nienhuis-Kuiper, M.; Dijkhuizen, L.; Appl. Environ. Microbiol. 74, 7197-7203 (2008)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodococcus erythropolis
B2ZFP6
terminal oxygenase homologue KshA2
-
Rhodococcus erythropolis
B5TYS8
KshA3
-
Rhodococcus erythropolis SQ1
B2ZFP6
terminal oxygenase homologue KshA2
-
Rhodococcus erythropolis SQ1
B5TYS8
KshA3
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-androstene-3,17-dione + O2 + NADH + H+
-
717119
Rhodococcus erythropolis
9alpha-hydroxyandrosta-4-en-3,17-dione + H2O + NAD+
-
-
-
ir
4-androstene-3,17-dione + O2 + NADH + H+
-
717119
Rhodococcus erythropolis SQ1
9alpha-hydroxyandrosta-4-en-3,17-dione + H2O + NAD+
-
-
-
ir
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717119
Rhodococcus erythropolis
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
ir
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717119
Rhodococcus erythropolis SQ1
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
ir
Cofactor
Cofactor
Commentary
Organism
Structure
[2Fe-2S]-center
;
Rhodococcus erythropolis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
[2Fe-2S]-center
-
Rhodococcus erythropolis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-androstene-3,17-dione + O2 + NADH + H+
-
717119
Rhodococcus erythropolis
9alpha-hydroxyandrosta-4-en-3,17-dione + H2O + NAD+
-
-
-
ir
4-androstene-3,17-dione + O2 + NADH + H+
-
717119
Rhodococcus erythropolis SQ1
9alpha-hydroxyandrosta-4-en-3,17-dione + H2O + NAD+
-
-
-
ir
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717119
Rhodococcus erythropolis
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
ir
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717119
Rhodococcus erythropolis SQ1
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
ir
Expression
Organism
Commentary
Expression
Rhodococcus erythropolis
expression of kshA2 is induced in parent strain Rhodococcus erythropolis SQ1 in the presence of androsta-1,4-diene-3,17-dione or 9alpha-hydroxy-4-androstene-3,17-dione or a metabolite thereof
up
General Information
General Information
Commentary
Organism
malfunction
gene deletion of kshA2 does not affect growth on 9alpha-hydroxy-4-androstene-3,17-dione, cholesterol, or cholic acid
Rhodococcus erythropolis
physiological function
3-ketosteroid 9alpha-hydroxylase is a key enzyme in microbial steroid degradation in Rhodococcus erythropolis strain SQ1 and is comprised of the terminal oxygenase component KshA1 and the oxygenase-reductase component KshB, a terminal oxygenase homologue KshA2 and homologue KshA3. Component KshA2 plays a role in preventing accumulation of toxic intracellular concentrations of 1,4-androstadiene-3,17-dione during steroid catabolism; 3-ketosteroid 9alpha-hydroxylase is a key enzyme in microbial steroid degradation in Rhodococcus erythropolis strain SQ1 and is comprised of the terminal oxygenase component KshA1 and the oxygenase-reductase component KshB, a terminal oxygenase homologue KshA2 and homologue KshA3. Component KshA2 plays a role in preventing accumulation of toxic intracellular concentrations of 1,4-androstadiene-3,17-dione during steroid catabolism
Rhodococcus erythropolis
General Information (protein specific)
General Information
Commentary
Organism
malfunction
gene deletion of kshA2 does not affect growth on 9alpha-hydroxy-4-androstene-3,17-dione, cholesterol, or cholic acid
Rhodococcus erythropolis
physiological function
3-ketosteroid 9alpha-hydroxylase is a key enzyme in microbial steroid degradation in Rhodococcus erythropolis strain SQ1 and is comprised of the terminal oxygenase component KshA1 and the oxygenase-reductase component KshB, a terminal oxygenase homologue KshA2 and homologue KshA3. Component KshA2 plays a role in preventing accumulation of toxic intracellular concentrations of 1,4-androstadiene-3,17-dione during steroid catabolism
Rhodococcus erythropolis
Expression (protein specific)
Organism
Commentary
Expression
Rhodococcus erythropolis
expression of kshA2 is induced in parent strain Rhodococcus erythropolis SQ1 in the presence of androsta-1,4-diene-3,17-dione or 9alpha-hydroxy-4-androstene-3,17-dione or a metabolite thereof
up
Other publictions for EC 1.14.15.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
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1
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1
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3
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1
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1
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1
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1
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744058
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3-Ketosteroid 9alpha-hydroxyl ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Mycolicibacterium smegmatis, Mycolicibacterium smegmatis mc2 155, Rhodococcus erythropolis, Rhodococcus erythropolis SQ1, Rhodococcus jostii, Rhodococcus rhodochrous, Rhodococcus rhodochrous DSM 43269
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-
2
2
-
1
-
-
-
-
5
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22
-
15
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-
-
5
-
-
-
-
28
5
-
-
-
-
-
-
-
10
-
-
-
-
2
4
14
-
1
-
-
-
-
-
-
7
-
22
-
-
-
-
-
-
-
-
28
7
-
-
-
-
-
-
-
-
-
20
28
-
-
-
745308
Penfield
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289
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1
2
-
-
4
19
-
-
-
8
-
18
-
-
1
2
-
-
-
-
54
2
2
-
-
19
2
-
-
-
3
-
-
-
-
2
-
3
-
-
-
6
3
20
-
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-
8
-
-
-
2
-
-
-
-
54
3
3
-
-
19
3
-
-
-
-
4
6
-
17
17
745698
Yeh
Deletion of the gene encoding ...
Rhodococcus hoagii 103S, Rhodococcus hoagii
Microb. Cell Fact.
13
130
2014
-
-
1
-
1
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
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-
-
-
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1
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-
1
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4
-
-
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-
-
-
-
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4
-
1
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-
-
-
-
1
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-
-
717737
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J. Bacteriol.
194
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-
-
1
-
8
-
-
2
-
1
-
3
-
13
-
-
1
-
-
-
-
-
74
-
-
-
-
-
1
-
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2
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-
-
-
3
4
-
16
-
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-
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4
-
2
-
3
-
-
-
3
-
-
-
-
74
-
-
-
-
-
2
-
-
-
-
1
2
-
-
-
717735
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193
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-
-
1
-
1
-
-
-
-
-
-
4
-
19
-
-
1
-
-
1
-
-
75
-
-
-
-
-
1
-
-
1
-
-
-
-
-
6
5
-
5
-
-
-
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-
-
-
-
4
-
-
-
6
-
3
-
-
75
-
-
-
-
-
3
-
-
-
1
3
11
5
-
-
717854
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Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
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286
40717-40724
2011
-
-
-
-
-
-
-
13
-
-
-
2
-
9
-
-
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-
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30
1
1
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-
11
1
-
-
1
-
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1
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-
-
-
-
21
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2
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-
-
-
-
-
-
-
30
2
2
-
-
19
2
-
-
-
-
2
3
-
13
19
713784
Wei
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Mycolicibacterium neoaurum, Mycolicibacterium neoaurum NwIB-01
Appl. Biochem. Biotechnol.
162
1446-1456
2010
-
-
-
-
-
-
-
-
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1
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3
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2
1
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1
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1
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2
1
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1
-
1
1
-
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-
716250
Hu
3-Ketosteroid 9alpha-hydroxyla ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Mol. Microbiol.
75
107-121
2010
-
-
-
-
-
-
-
-
-
-
-
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-
160
-
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4
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4
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-
2
2
-
-
-
717500
Fan
Cloning, heterologous expressi ...
Mycobacterium sp., Mycobacterium sp. NwIB-01
Chin. J. Biotechnol.
25
2014-2021
2009
-
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1
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1
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8
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1
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1
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1
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1
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1
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-
1
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-
-
-
-
-
-
-
-
-
-
717784
Capyk
Characterization of 3-ketoster ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Biol. Chem.
284
9937-9946
2009
-
-
1
1
-
-
-
3
-
-
-
-
-
6
-
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1
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4
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3
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1
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1
1
1
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3
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1
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4
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3
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-
-
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-
-
-
3
3
717119
Van Der Geize
Characterization of a second R ...
Rhodococcus erythropolis, Rhodococcus erythropolis SQ1
Appl. Environ. Microbiol.
74
7197-7203
2008
-
-
-
-
-
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-
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9
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8
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8
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1
2
3
1
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717118
Andor
Generation of useful insertion ...
Mycolicibacterium smegmatis, Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
Appl. Environ. Microbiol.
72
6554-6559
2006
-
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1
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1
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2
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718125
Van Der Geize
Molecular and functional chara ...
Rhodococcus erythropolis, Rhodococcus erythropolis SQ1
Mol. Microbiol.
45
1007-1018
2002
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1
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1
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4
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2
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8
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