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Literature summary for 1.14.15.3 extracted from

  • Al-Hawash, A.B.; Zhang, J.; Li, S.; Liu, J.; Ghalib, H.B.; Zhang, X.; Ma, F.
    Biodegradation of n-hexadecane by Aspergillus sp. RFC-1 and its mechanism (2018), Ecotoxicol. Environ. Saf., 164, 398-408.
    View publication on PubMed
Search for more literature for 1.14.15.3

Cloned(Commentary)

Cloned (Comment) Organism
quantitative RT-PCR enzyme expression analysis Aspergillus sp. RFC-1

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ in the cytochrome P450 heme Aspergillus sp. RFC-1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
octane + 2 reduced rubredoxin + O2 + 2 H+ Aspergillus sp. RFC-1
-
 1-octanol + 2 oxidized rubredoxin + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus sp. RFC-1
-
 isolated from oil-contaminated soil
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
n-hexadecane + 2 reduced rubredoxin + O2 + 2 H+ during 10 days of incubation, 86.3% of HXD is degraded by strain RFC-1 Aspergillus sp. RFC-1 1-hexadecanol + 2 oxidized rubredoxin + H2O
-
 ?
octane + 2 reduced rubredoxin + O2 + 2 H+
-
 Aspergillus sp. RFC-1 1-octanol + 2 oxidized rubredoxin + H2O
-
 ?

Synonyms

Synonyms Comment Organism
alkane hydroxylase
-
 Aspergillus sp. RFC-1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Aspergillus sp. RFC-1

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
 Aspergillus sp. RFC-1
Ferredoxin with ferredoxin reductase Aspergillus sp. RFC-1
heme
-
 Aspergillus sp. RFC-1
additional information electrons are transferred from NADH to the cofactors Aspergillus sp. RFC-1
rubredoxin
-
 Aspergillus sp. RFC-1

Expression

Organism Comment Expression
Aspergillus sp. RFC-1 the expression level of the cytochrome P450 gene encoding the enzyme is induced from 0.94fold to 5.45fold under the n-hexadecane (HXD) condition up

General Information

General Information Comment Organism
physiological function the enzyme from Aspergillus sp. RFC-1 catalyzes the biodegradation of n-hexadecane (HXD). Results of blood agar plate and thin-layer chromatography analysis show that strain RFC-1 releases high lipid and emulsification activity in the fungal culture. Induced cell surface hydrophobicity and reduced surface tension also indicate the RFC-1-mediated biosurfactant production, which facilitates the HXD degradation and supported the degradation process. Reaction mode and cell phenotype, overview Aspergillus sp. RFC-1