Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.15.3 extracted from

  • Tsai, Y.F.; Luo, W.I.; Chang, J.L.; Chang, C.W.; Chuang, H.C.; Ramu, R.; Wei, G.T.; Zen, J.M.; Yu, S.S.
    Electrochemical hydroxylation of C3-C12 n-alkanes by recombinant alkane hydroxylase (AlkB) and rubredoxin-2 (AlkG) from Pseudomonas putida GPo1 (2017), Sci. Rep., 7, 8369 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.15.3

Application

Application Comment Organism
synthesis electrocatalytic conversion from alkanes to alcohols mediated by AlkB using a rubredoxin AlkG immobilized screen-printed carbon electrode. As electrons can be transferred from the reduced AlkG to AlkB in a two-phase manner, electrocatalytic conversion from alkanes to alcohols can be applied. Use for conversion of gaseous propane and n-butane to 1-propanol and 1-butanol, respectively Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida GPo1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
n-butane + reduced rubredoxin + O2
-
 Pseudomonas putida 1-butanol + oxidized rubredoxin + H2O
-
 ?
n-butane + reduced rubredoxin + O2
-
 Pseudomonas putida GPo1 1-butanol + oxidized rubredoxin + H2O
-
 ?
n-octane + reduced rubredoxin + O2
-
 Pseudomonas putida n-octanol + oxidized rubredoxin + H2O
-
 ?
n-octane + reduced rubredoxin + O2
-
 Pseudomonas putida GPo1 n-octanol + oxidized rubredoxin + H2O
-
 ?
n-propane + reduced rubredoxin + O2
-
 Pseudomonas putida 1-propanol + oxidized rubredoxin + H2O
-
 ?
n-propane + reduced rubredoxin + O2
-
 Pseudomonas putida GPo1 1-propanol + oxidized rubredoxin + H2O
-
 ?

General Information

General Information Comment Organism
physiological function one rubredoxin AlkG with two Fe-4S electron transfer centres can bind with two membrane-bound AlkB to form an AlkG:2AlkB adduct, facilitating an efficient conversion of n-octane to 1-octanol. the specific activity among all chain length linear alkanes tested varies, there is no particular relationship between the specific activity and the chain length of alkanes Pseudomonas putida