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Literature summary for 1.14.15.3 extracted from

  • Dierks, E.A.; Davis, S.C.; Ortiz de Montellano, P.R.
    Glu-325 and Asp-328 are determinants of the cyp4A1 hydroxylation regiospecificity and resistance to inactivation by 1-aminobenzotriazole (1998), Biochemistry, 37, 1839-1847.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli XL-1 blue, human gene CYP4A1 Homo sapiens

Protein Variants

Protein Variants Comment Organism
D323E gene CYP4A1 Homo sapiens
E320A gene CYP4A1 Homo sapiens
E320A/D323E gene CYP4A1 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
lauric acid D323E mutant Homo sapiens
0.031
-
lauric acid E320A/D323E mutant Homo sapiens
0.036
-
lauric acid E320A mutant Homo sapiens
0.16
-
lauric acid
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
arachidonic acid + NADPH + H+ + O2 Homo sapiens
-
20-hydroxyeicosatetraenoic acid + NADP+ + H2O
-
?
n-alkane + NAD(P)H + H+ + O2 Homo sapiens
-
n-alkanol + NAD(P)+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonic acid + NADPH + H+ + O2
-
Homo sapiens 20-hydroxyeicosatetraenoic acid + NADP+ + H2O
-
?
lauric acid + NAD(P)H + H+ + O2
-
Homo sapiens 12-hydroxydodecanoic acid + NAD(P)+ + H2O
-
?
n-alkane + NAD(P)H + H+ + O2
-
Homo sapiens n-alkanol + NAD(P)+ + H2O
-
?