Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli HMS174 (DE3) cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using MgCl2 with 0.1 M HEPES, pH 7.0 or 7.5, and 20% (w/v) PEG 6000 or 25% (w/v) PEG 3350, respectively | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography, Resource-Q column chromatography, and Sephacryl S-200 gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Mycobacterium tuberculosis | 27-hydroxycholesterol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP125 | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Mycobacterium tuberculosis | |
NADPH | - |
Mycobacterium tuberculosis |