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Literature summary for 1.14.15.29 extracted from

  • McLean, K.; Lafite, P.; Levy, C.; Cheesman, M.; Mast, N.; Pikuleva, I.; Leys, D.; Munro, A.
    The structure of Mycobacterium tuberculosis CYP125: Molecular basis for cholesterol binding in a P450 needed for host infection (2009), J. Biol. Chem., 284, 35524-35533.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli HMS174 (DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using MgCl2 with 0.1 M HEPES, pH 7.0 or 7.5, and 20% (w/v) PEG 6000 or 25% (w/v) PEG 3350, respectively Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography, Resource-Q column chromatography, and Sephacryl S-200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Mycobacterium tuberculosis 27-hydroxycholesterol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

Synonyms Comment Organism
CYP125
-
 Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
heme
-
 Mycobacterium tuberculosis
NADPH
-
 Mycobacterium tuberculosis