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Literature summary for 1.14.15.25 extracted from

  • Dutta, T.; Dutta, A.; Chakraborty, J.; Sarkar, J.; Pal Chowdhury, P.; Gunsalus, I.
    Purification and properties of reductase of the three-component p-cymene methyl hydroxylase from Pseudomonas chlororaphis subsp. aureofaciens (2012), Process Biochem., 47, 1263-1267 .
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Cloned(Commentary)

Cloned (Comment) Organism
-
 Pseudomonas chlororaphis subsp. aureofaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 gel fitlration, reductase component CymA Pseudomonas chlororaphis subsp. aureofaciens

Organism

Organism UniProt Comment Textmining
Pseudomonas chlororaphis subsp. aureofaciens P95461 and O84920 and O84919 P95461 i.e. reductase subunit CymA, cf. EC 1.8.1.3, O84920 i.e. subunit CymB, O84919 i.e. subunit CymM
-

Storage Stability

Storage Stability Organism
70°C, 50 mM potassium phosphate buffer (pH 7.0), 60% glycerol (v/v), subunit CymA is stable for 1 month Pseudomonas chlororaphis subsp. aureofaciens

Subunits

Subunits Comment Organism
? x * 38433, calculated, x * 38000, SDS-PAGE of reductase component CymA Pseudomonas chlororaphis subsp. aureofaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 10 min, 42% loss of activity, NADH-cytochrome c reductase activity of subunit CymA Pseudomonas chlororaphis subsp. aureofaciens
50
-
 10 min, 98% loss of activity, NADH-cytochrome c reductase activity of subunit CymA Pseudomonas chlororaphis subsp. aureofaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
 NADH-cytochrome c reductase activity of subunit CymA Pseudomonas chlororaphis subsp. aureofaciens

Expression

Organism Comment Expression
Pseudomonas chlororaphis subsp. aureofaciens expression of subunits CymABM is induced by growth on p-cymene up