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Literature summary for 1.14.15.25 extracted from

  • Nishio, T.; Patel, A.; Wang, Y.; Lau, P.C.
    Biotransformations catalyzed by cloned p-cymene monooxygenase from Pseudomonas putida F1 (2001), Appl. Microbiol. Biotechnol., 55, 321-325 .
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida O33456 and O33457 O33456 i.e. subunit CymAa, O33457 i.e. subunit CymAb
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-(methylthio)toluene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida 4-(methylthio)benzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
4-chlorostyrene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida 4-chlorostyrene oxide + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
4-ethyltoluene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida 4-ethylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
4-fluorotoluene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida 4-fluorobenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
p-cymene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida 4-isopropylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
styrene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida styrene oxide + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Cofactor

Cofactor Comment Organism Structure
Ferredoxin
-
 Pseudomonas putida