BRENDA - Enzyme Database
show all sequences of 1.14.15.24

Cloning and characterization of a novel beta-carotene hydroxylase gene from Lycium barbarum and its expression in Escherichia coli

Wu, J.; Ji, J.; Wang, G.; Li, Z.; Diao, J.; Wu, G.; Biotechnol. Appl. Biochem. 61, 637-645 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Lycium barbarum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Lycium barbarum
9507
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Lycium barbarum
-
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lycium barbarum
U5Y6R4
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography
Lycium barbarum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
-
Lycium barbarum
-
fruit
highest expression
Lycium barbarum
-
leaf
-
Lycium barbarum
-
root
-
Lycium barbarum
-
stem
lowest expression
Lycium barbarum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
744536
Lycium barbarum
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 34800, calculated from amino acid sequence; x * 37000, SDS-PAGE
Lycium barbarum
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Lycium barbarum
calculated from amino acid sequence
-
8.4
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Lycium barbarum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Lycium barbarum
9507
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Lycium barbarum
-
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography
Lycium barbarum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
-
Lycium barbarum
-
fruit
highest expression
Lycium barbarum
-
leaf
-
Lycium barbarum
-
root
-
Lycium barbarum
-
stem
lowest expression
Lycium barbarum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
744536
Lycium barbarum
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 34800, calculated from amino acid sequence; x * 37000, SDS-PAGE
Lycium barbarum
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Lycium barbarum
calculated from amino acid sequence
-
8.4
Other publictions for EC 1.14.15.24
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742396
Zhou
Alleviation of metabolic bott ...
Haematococcus lacustris
Enzyme Microb. Technol.
100
28-36
2017
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1
1
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742573
Wang
-
Engineering of beta-carotene ...
Alcaligenes sp. PC-1
Front. Chem. Sci. Engin.
11
89-99
2017
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1
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743205
Lu
Metabolic engineering of Esch ...
Pantoea ananatis
Mar. Drugs
15
E296
2017
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1
1
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1
1
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746018
Zunjare
-
Influence of rare alleles of ...
Zea mays
Plant Breed.
136
872-880
2017
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746128
Kang
Suppression of the beta-carot ...
Ipomoea batatas
Plant Physiol. Biochem.
117
24-33
2017
-
-
-
-
-
-
-
-
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1
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1
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1
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1
1
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749390
Kildegaard
Engineering of Yarrowia lipol ...
Pantoea ananatis
Synth. Syst. Biotechnol.
2
287-294
2017
-
1
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1
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743982
Simon
-
Heterologous expression of be ...
Chlamydomonas reinhardtii
Algal Res.
18
257-265
2016
-
-
1
-
-
-
-
-
-
-
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1
-
1
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745622
Choudhary
-
Microsatellite marker-based g ...
Zea mays
J. Plant Biochem. Biotechnol.
25
12-20
2016
-
-
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1
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1
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1
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745670
Henke
Production of the marine caro ...
Fulvimarina pelagi
Mar. Drugs
14
124
2016
-
-
1
-
-
-
-
-
-
-
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3
-
1
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744519
Chang
Integrating an algal beta-car ...
Chlamydomonas reinhardtii, Chromochloris zofingiensis, Haematococcus lacustris
Biores. Technol.
184
2-8
2015
-
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3
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3
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3
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746019
Wu
-
Ectopic expression of the Lyc ...
Lycium barbarum
Plant Cell
121
559-569
2015
-
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1
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3
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1
1
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746142
Kilcrease
Correlations of carotenoid co ...
Capsicum annuum
Plant Sci.
232
57-66
2015
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3
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744536
Wu
Cloning and characterization ...
Lycium barbarum
Biotechnol. Appl. Biochem.
61
637-645
2014
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1
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5
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5
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1
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1
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745620
Zhao
-
Over-expression of Arabidopsi ...
Arabidopsis thaliana
J. Plant Biochem. Biotechnol.
23
190-198
2014
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1
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1
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728824
Borovsky
Induced mutation in beta-CAROT ...
Capsicum annuum
Theor. Appl. Genet.
126
557-565
2013
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1
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720286
Scaife
Comparative analysis of ?-caro ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
J. Nat. Prod.
75
1117-1124
2012
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727255
Fiore
A quadruple mutant of Arabidop ...
Arabidopsis thaliana
BMC Plant Biol.
12
50
2012
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1
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728445
Kim
Down-regulation of beta-carote ...
Ipomoea batatas
Phytochemistry
74
69-78
2012
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1
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728463
Lv
Rice carotenoid beta-ring hydr ...
Oryza sativa
Plant Cell Physiol.
53
987-1002
2012
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1
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2
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728518
Quinlan
Synergistic interactions betwe ...
Oryza sativa
Plant Physiol.
160
204-214
2012
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1
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3
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716971
D'Ambrosio
Overexpression of CrtR-b2 (car ...
Solanum lycopersicum
Transgenic Res.
20
47-60
2011
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728232
Misawa
Carotenoid beta-ring hydroxyla ...
Brevundimonas sp., Paracoccus sp.
Mar. Drugs
9
757-771
2011
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2
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2
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728489
Zhao
-
Molecular cloning and expressi ...
Diospyros kaki
Plant Mol. Biol. Rep.
29
345-351
2011
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Over-expression of Arabidopsis ...
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The evolution and function of ...
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748597
Martin
Conversion of beta-carotene i ...
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Yu
Characterization of a beta-car ...
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673817
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The crtS gene of Xanthophyllom ...
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713863
Choi
Characterization of bacterial ...
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40
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714018
Momoi
Reconstitution of beta-caroten ...
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2006
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Fiore
Elucidation of the beta-carote ...
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Ojima
Cloning of the astaxanthin syn ...
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747895
Tao
A carotenoid synthesis gene c ...
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Blasco
CYP175A1 from Thermus thermoph ...
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Progress in understanding the ...
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Inoue
Carotenoid hydroxylation - P45 ...
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2004
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714301
Zhu
cDNAs for the synthesis of cyc ...
Gentiana lutea
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1625
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Tian
Functional analysis of beta- a ...
Arabidopsis thaliana
Plant Cell
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10
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716322
Davison
Overexpression of beta-caroten ...
Arabidopsis thaliana
Nature
418
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2002
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3
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Rissler
Antisense inhibition of the be ...
Arabidopsis thaliana
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Steinbrenner
Regulation of two carotenoid b ...
Haematococcus lacustris, Haematococcus lacustris Flotow NIES-144
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125
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2001
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Linden
Carotenoid hydroxylase from Ha ...
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Bouvier
Xanthophyll biosynthesis: Mole ...
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Fraser
Enzymic confirmation of reacti ...
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