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Literature summary for 1.14.15.24 extracted from

  • Sun, Z.; Gantt, E.; Cunningham, F.X.
    Cloning and functional analysis of the beta-carotene hydroxylase of Arabidopsis thaliana (1996), J. Biol. Chem., 271, 24349-24352.
    View publication on PubMed
Search for more literature for 1.14.15.24

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH10BZIP cells Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34400
-
 2 * 34400, calculated from amino acid sequence Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9SZZ8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Arabidopsis thaliana beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
-
 Arabidopsis thaliana zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?
beta-zeacarotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2 beta-zeacarotene is 7',8'-dihydro-beta,psi-carotene Arabidopsis thaliana hydroxy-beta-zeacarotene + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O hydroxy-beta-zeacarotene is 7',8'-dihydro-beta,psi-carotene-3-ol ?
additional information the epsilon rings of delta-carotene (epsilon,psi-carotene) and alpha-zeacarotene(7',8'-dihydro-epsilon,psi-carotene) are poor substrates for the enzyme Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 34400, calculated from amino acid sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
beta-carotene hydroxylase
-
 Arabidopsis thaliana
CrtZ
-
 Arabidopsis thaliana

pI Value

Organism Comment pI Value Maximum pI Value
Arabidopsis thaliana calculated from amino acid sequence
-
 9.4