Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO | - |
Mus musculus | |
ketoconazole | - |
Mus musculus | |
Metyrapone | - |
Mus musculus | |
vitamin D3 | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
24,25-dihydroxycholecalciferol | - |
Mus musculus | |
0.0027 | - |
25-hydroxycholecalciferol | - |
Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
25-hydroxycholecalciferol + NADPH + O2 | Mus musculus | recombinant in E. coli | calcitriol + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O | cytochrome P-450 enzyme | Mus musculus | |
calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O | reconstituted with cytochrome P-450, adrenal ferredoxin, i.e. adrenodoxin, and adrenodoxin reductase | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000002 | - |
substrate 23,25-dihydroxycholecalciferol | Mus musculus |
0.0000011 | - |
substrate 24-oxo-25-hydroxycholecalciferol | Mus musculus |
0.0000015 | - |
- |
Mus musculus |
0.0000043 | - |
substrate 24,25-dihydroxycholecalciferol | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(24R),25-dihydroxycholecalciferol + NADPH + O2 | recombinant enzyme | Mus musculus | 1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O | - |
? | |
(24R),25-dihydroxycholecalciferol + NADPH + O2 | higher activity than with 25-hydroxycholecalciferol | Mus musculus | 1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O | - |
? | |
23,25-dihydroxycholecalciferol + NADPH + O2 | low activity | Mus musculus | ? | - |
? | |
23,25-dihydroxycholecalciferol + NADPH + O2 | recombinant enzyme | Mus musculus | ? | - |
? | |
24,25-dihydroxycholecalciferol + reduced adrenodoxin + O2 | - |
Mus musculus | 1alpha,24,25-trihydroxycholecalciferol + oxidized adrenodoxin + H2O | - |
r | |
24-oxo-23,25-dihydroxycholecalciferol + NADPH + O2 | low activity | Mus musculus | ? | - |
? | |
24-oxo-23,25-dihydroxycholecalciferol + NADPH + O2 | recombinant enzyme | Mus musculus | ? | - |
? | |
24-oxo-25-hydroxycholecalciferol + NADPH + O2 | recombinant enzyme | Mus musculus | ? | - |
? | |
25-hydroxycholecalciferol + NADPH + O2 | recombinant in E. coli | Mus musculus | calcitriol + NADP+ + H2O | - |
? | |
calcidiol + NADPH + O2 | recombinant enzyme | Mus musculus | calcitriol + NADP+ + H2O | calcitriol is identical with 1,25-dihydroxycholecalciferol | ? | |
calcidiol + NADPH + O2 | highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol | Mus musculus | calcitriol + NADP+ + H2O | calcitriol is identical with 1,25-dihydroxycholecalciferol | ? | |
additional information | the 25-hydroxy group is essential for 1alpha-hydroxylating activity, the 24-hydroxy group enhances the activity, the 23-hydroxy group strongly reduces activity | Mus musculus | ? | - |
? | |
additional information | no activity with 24,25,26,27-tetranor-23-hydroxyvitamin D3 and vitamin D3 | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYB27B1 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Mus musculus |