Literature summary for 1.14.15.11 extracted from

Xu, L.; Ikeda, H.; Liu, L.; Arakawa, T.; Wakagi, T.; Shoun, H.; Fushinobu, S.
Structural basis for the 4'-hydroxylation of diclofenac by a microbial cytochrome P450 monooxygenase (2015), Appl. Microbiol. Biotechnol., 99, 3081-3091 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Streptomyces avermitilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with diclofenac, at 2.2 A resolution. The distal pocket of CYP105D7 contains two diclofenac molecules. The C3' and C4' atoms of the dichlorophenyl ring of one diclofenac molecule are positioned near the heme iron	Streptomyces avermitilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	diclofenac	CYP105D7 binds diclofenac in a slightly cooperative manner with an affinity of 65 microM and a Hill coefficient of 1.16, pH not specified in the publication, temperature not specified in the publication	Streptomyces avermitilis

Organism

Organism	UniProt	Comment	Textmining
Streptomyces avermitilis	Q825I8	-	-
Streptomyces avermitilis DSM 46492	Q825I8	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-deoxypentalenic acid + reduced ferredoxin + O2	-	Streptomyces avermitilis	pentalenic acid + oxidized ferredoxin + H2O	-	?
1-deoxypentalenic acid + reduced ferredoxin + O2	-	Streptomyces avermitilis DSM 46492	pentalenic acid + oxidized ferredoxin + H2O	-	?
diclofenac + reduced ferredoxin + O2	-	Streptomyces avermitilis	4'-hydroxydiclofenac + oxidized ferredoxin + H2O	-	?
diclofenac + reduced ferredoxin + O2	-	Streptomyces avermitilis DSM 46492	4'-hydroxydiclofenac + oxidized ferredoxin + H2O	-	?

Subunits

Subunits	Comment	Organism
?	x * 45000, SDS-PAGE, recombinant protein with His-tag	Streptomyces avermitilis

Synonyms

Synonyms	Comment	Organism
CYP105D7	-	Streptomyces avermitilis
pentalenic acid synthase	-	Streptomyces avermitilis

Cofactor

Cofactor	Comment	Organism	Structure
heme	-	Streptomyces avermitilis

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Release 2023.1 (January 2023)