BRENDA - Enzyme Database
show all sequences of 1.14.15.10

P450camr, a cytochrome P450 catalysing the stereospecific 6-endo-hydroxylation of (1R)-(+)-camphor

Grogan, G.; Roberts, G.; Parsons, S.; Turner, N.; Flitsch, S.; Appl. Microbiol. Biotechnol. 59, 449-454 (2002)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Rhodococcus sp.
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Rhodococcus sp.
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
x * 44000, SDS-PAGE
Rhodococcus sp.
Organism
Organism
UniProt
Commentary
Textmining
Rhodococcus sp.
-
-
-
Rhodococcus sp. NCIMB 9784
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(+)-camphor + reduced putidaredoxin + O2
-
717127
Rhodococcus sp.
(+)-6-endo-hydroxycamphor + oxidized putidaredoxin + H2O
absolute stereospecificity for endo-hydroxylation
-
-
?
(+)-camphor + reduced putidaredoxin + O2
-
717127
Rhodococcus sp. NCIMB 9784
(+)-6-endo-hydroxycamphor + oxidized putidaredoxin + H2O
absolute stereospecificity for endo-hydroxylation
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 44000, SDS-PAGE
Rhodococcus sp.
Synonyms
Synonyms
Commentary
Organism
P450camr
-
Rhodococcus sp.
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Rhodococcus sp.
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Rhodococcus sp.
isoelectric focusing
-
4.8
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Rhodococcus sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Rhodococcus sp.
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Rhodococcus sp.
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
x * 44000, SDS-PAGE
Rhodococcus sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(+)-camphor + reduced putidaredoxin + O2
-
717127
Rhodococcus sp.
(+)-6-endo-hydroxycamphor + oxidized putidaredoxin + H2O
absolute stereospecificity for endo-hydroxylation
-
-
?
(+)-camphor + reduced putidaredoxin + O2
-
717127
Rhodococcus sp. NCIMB 9784
(+)-6-endo-hydroxycamphor + oxidized putidaredoxin + H2O
absolute stereospecificity for endo-hydroxylation
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 44000, SDS-PAGE
Rhodococcus sp.
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Rhodococcus sp.
isoelectric focusing
-
4.8
Expression
Organism
Commentary
Expression
Rhodococcus sp.
induced by growth on (1R)-(+)-camphor
up
Expression (protein specific)
Organism
Commentary
Expression
Rhodococcus sp.
induced by growth on (1R)-(+)-camphor
up
Other publictions for EC 1.14.15.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
717127
Grogan
P450camr, a cytochrome P450 ca ...
Rhodococcus sp., Rhodococcus sp. NCIMB 9784
Appl. Microbiol. Biotechnol.
59
449-454
2002
-
-
1
-
-
-
-
-
1
-
1
-
-
3
-
-
-
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
1
-
1
-
-
-
1
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
1
-
-
1
-
-