Cloned (Comment) | Organism |
---|---|
ArR/Arx electron-transport chain co-expressed with the CYP enzymes in Escherichia coli. Amplified genes incorporated into the pRSFDuet-Arx vector using the Nde I and Kpn I/EcoR V restriction sites, plasmids pETDuet-Arx-ArR and one of the P450 expressing plasmids pRSFDuet-Arx-CYP transformed into Escherichia coli competent BL21(DE3) cells | Novosphingobium aromaticivorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Novosphingobium aromaticivorans | - |
strain ATCC 700278D-5 | - |
Storage Stability | Organism |
---|---|
-20°C, 50 mM Tris buffer, pH 7.4, 50% v/v glycerol | Novosphingobium aromaticivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-camphor + O2 + reduced putidaredoxin | CYP101D1, CYP101D2 | Novosphingobium aromaticivorans | (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
(+)-camphor + O2 + reduced putidaredoxin | CYP101D1, CYP101D2 | Novosphingobium aromaticivorans ATCC 700278D-5 | (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
2-adamantanone + O2 + reduced putidaredoxin | CYP101D1 and CYP101D2 | Novosphingobium aromaticivorans | 5-hydroxy-2-adamantanone + oxidized putidaredoxin + H2O | - |
? | |
2-adamantanone + O2 + reduced putidaredoxin | CYP101D1 and CYP101D2 | Novosphingobium aromaticivorans ATCC 700278D-5 | 5-hydroxy-2-adamantanone + oxidized putidaredoxin + H2O | - |
? | |
beta-ionone + O2 + reduced putidaredoxin | CYP101C1 and CYP101B1. CYP101C1 oxidizes beta-ionone to 4-hydroxy-beta-ionone (75%) with one other, unidentified product. This latter compound is the major product of beta-ionone oxidation by CYP101B1 (90%) where 4-hydroxy-beta-ionone is the minor product (10%) | Novosphingobium aromaticivorans | 4-hydroxy-beta-ionone + oxidized putidaredoxin + H2O | - |
? | |
beta-ionone + O2 + reduced putidaredoxin | CYP101C1 and CYP101B1. CYP101C1 oxidizes beta-ionone to 4-hydroxy-beta-ionone (75%) with one other, unidentified product. This latter compound is the major product of beta-ionone oxidation by CYP101B1 (90%) where 4-hydroxy-beta-ionone is the minor product (10%) | Novosphingobium aromaticivorans ATCC 700278D-5 | 4-hydroxy-beta-ionone + oxidized putidaredoxin + H2O | - |
? | |
linalool + O2 + reduced putidaredoxin | CYP111A2 and CYP111A1 | Novosphingobium aromaticivorans | 8-hydroxy-linalool + oxidized putidaredoxin + H2O | - |
? | |
linalool + O2 + reduced putidaredoxin | CYP111A2 and CYP111A1 | Novosphingobium aromaticivorans ATCC 700278D-5 | 8-hydroxy-linalool + oxidized putidaredoxin + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP101B1 | - |
Novosphingobium aromaticivorans |
CYP101C1 | - |
Novosphingobium aromaticivorans |
CYP101D1 | - |
Novosphingobium aromaticivorans |
CYP101D2 | - |
Novosphingobium aromaticivorans |
CYP111A2 | - |
Novosphingobium aromaticivorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
putidaredoxin | - |
Novosphingobium aromaticivorans |