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Literature summary for 1.14.15.1 extracted from

  • Hirao, H.; Kumar, D.; Shaik, S.
    On the identity and reactivity patterns of the "second oxidant" of the T252A mutant of cytochrome P450cam in the oxidation of 5-methylenenylcamphor (2006), J. Inorg. Biochem., 100, 2054-2068.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
T252A the mutant can epoxidize olefins like 5-methylenyl-camphor, but is ineffective in camphor hydroxylation Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Iron heme iron Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-camphor + O2 + reduced putidaredoxin Pseudomonas putida
-
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-camphor + O2 + reduced putidaredoxin
-
Pseudomonas putida (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
?
(+)-camphor + O2 + reduced putidaredoxin wild-type enzyme, but not mutant T252A Pseudomonas putida (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
?
5-methylenyl-camphor + O2 + reduced putidaredoxin wild-type enzyme and mutant T252A, in absence of the primary oxidant species of P450, the precursor species FeOOH can effect double bond activation of 5-methylenyl-camphor initiated by a homolytic cleavage of the O-O-bond and formation of an OH radical bound to the secondary oxidant by hydrogen bonding interaction, overview Pseudomonas putida ?
-
?

Synonyms

Synonyms Comment Organism
cytochrome p450cam
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
putidaredoxin
-
Pseudomonas putida