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Literature summary for 1.14.15.1 extracted from

  • Loida, P.J.; Sligar, S.G.
    Engineering cytochrome P-450cam to increase the stereospecificity and coupling of aliphatic hydroxylation (1993), Protein Eng., 6, 207-212.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
T101M ratio of (R)- to (S)-1-phenylethanol produced from ethylbenzene is 89:11 Pseudomonas putida
T101M/T185F/V247M ratio of (R)- to (S)-1-phenylethanol produced from ethylbenzene is 87:13 Pseudomonas putida
T185F ratio of (R)- to (S)-1-phenylethanol produced from ethylbenzene is 78:22 Pseudomonas putida
T185L ratio of (R)- to (S)-1-phenylethanol produced from ethylbenzene is 80:20 Pseudomonas putida
T185V ratio of (R)- to (S)-1-phenylethanol produced from ethylbenzene is 74:26 Pseudomonas putida
V247A ratio of (R)- to (S)-1-phenylethanol produced from ethylbenzene is 87:13 Pseudomonas putida
V247M ratio of (R)- to (S)-1-phenylethanol produced from ethylbenzene is 83:17 Pseudomonas putida
V295I ratio of (R)- to (S)-1-phenylethanol produced from ethylbenzene is 76:24 Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-camphor + putidaredoxin + O2 Pseudomonas putida
-
(R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
Accession Number PC00183
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-camphor + putidaredoxin + O2
-
Pseudomonas putida (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?
ethylbenzene + putidaredoxin + O2 at 5% of the reaction with (+)-camphor Pseudomonas putida 1-phenylethanol + oxidized putidaredoxin + H2O ratio of (R)- to (S)-1-phenylethanol produced depends on mutant form ?

Cofactor

Cofactor Comment Organism Structure
NADH
-
Pseudomonas putida