Cloned (Comment) | Organism |
---|---|
gene CYP82E4v1, DNA and amino acid sequence determination and analysis, real-time PCR enzyme expression analysis | Nicotiana tabacum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme silencing by CYP82E21-specific RNAi expression. CYP82E4v1-interference affects the accumulation of nicotine and nornicotine, and the RNAi vector for CYP82E4v1 effectively silences CYP82E4v1 expression and further blocks the synthesis of nornicotine | Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotine + NADPH + H+ | Nicotiana tabacum | - |
nornicotine + NADP+ | - |
? | |
nicotine + [reduced NADPH-hemoprotein reductase] + O2 | Nicotiana tabacum | - |
nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | Q38Q86 | var. K326 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Nicotiana tabacum | - |
root | highest expression level of the enzyme | Nicotiana tabacum | - |
stem | - |
Nicotiana tabacum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotine + NADPH + H+ | - |
Nicotiana tabacum | nornicotine + NADP+ | - |
? | |
nicotine + [reduced NADPH-hemoprotein reductase] + O2 | - |
Nicotiana tabacum | nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP82E4v1 | - |
Nicotiana tabacum |
nicotine N-demethylase | - |
Nicotiana tabacum |
NND | - |
Nicotiana tabacum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Nicotiana tabacum | |
NADPH | - |
Nicotiana tabacum |
General Information | Comment | Organism |
---|---|---|
malfunction | RNA interference of the nicotine demethylase gene CYP82E4v1 reduces nornicotine content and enhances Myzus persicae resistance in Nicotiana tabacum infected with aphids | Nicotiana tabacum |