Cloned (Comment) | Organism |
---|---|
gene CYP82E21, unrooted phylogenetic analysis and tree of CYP82E21 and related CYP82E coding sequences, quantitative PCR enzyme expression analysis, ectopic expression of the coding sequence in a tobacco line lacking functional CYP82E4, CYP82E5 and CYP82E10 genes, resulting in an eightfold increase of nicotine demethylation compared to the control plants confirming the nicotine N-demethylase activity of CYP82E21 | Nicotiana tabacum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme silencing by introducing a CYP82E21-specific RNAi construct in ovaries, silencing of CYP82E21 leads to up to 90% reduced nicotine conversion in flower ovaries | Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotine + NADPH + H+ | Nicotiana tabacum | - |
nornicotine + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
var. TN90 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | regarding sepals, petals, ovaries, stigma including style, filaments and anthers, CYP82E21 expression is found almost exclusively in ovaries, with very low expression levels observed in other flower organs | Nicotiana tabacum | - |
additional information | CYP82E21 expression pattern analysis, CYP82E21 is specifically expressed in ovaries but not expressed in leaves | Nicotiana tabacum | - |
ovary | specific for | Nicotiana tabacum | - |
plant ovary | specific for | Nicotiana tabacum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotine + NADPH + H+ | - |
Nicotiana tabacum | nornicotine + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP82E21 | - |
Nicotiana tabacum |
nicotine N-demethylase | - |
Nicotiana tabacum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Nicotiana tabacum | |
NADPH | - |
Nicotiana tabacum |
General Information | Comment | Organism |
---|---|---|
evolution | N-demethylase enzymes (NND) belongs to the family of cytochrome P450 monooxygenases (CYP). The CYP82E21 gene is found in all Nicotiana tabacum cultivars analysed and originates from the tobacco ancestor Nicotiana tomentosiformis | Nicotiana tabacum |
malfunction | nornicotine formation can be reduced in ovaries by introducing a CYP82E21-specific RNAi construct | Nicotiana tabacum |
physiological function | the CYP82E21 gene encodes a functional ovary-specific nicotine N-demethylase, nornicotine is formed by oxidative demethylation of nicotine | Nicotiana tabacum |