Literature summary for 1.14.14.B11 extracted from

Pakdeechanuan, P.; Teoh, S.; Shoji, T.; Hashimoto, T.
Non-functionalization of two CYP82E nicotine N-demethylase genes abolishes nornicotine formation in nicotiana langsdorffii (2012), Plant Cell Physiol., 53, 2038-2046.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Nicotiana alata
-	Nicotiana langsdorffii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0203	-	(S)-nicotine	isoform CYP82E1, in 50 mM Tris-HCl (pH 7.5), at 25°C	Nicotiana alata
0.054	-	(S)-nicotine	isoform CYP82E2, in 50 mM Tris-HCl (pH 7.5), at 25°C	Nicotiana alata
0.0619	-	(S)-nicotine	isoform CYP82E4, in 50 mM Tris-HCl (pH 7.5), at 25°C	Nicotiana alata
0.0684	-	(S)-nicotine	isoform CYP82E1, in 50 mM Tris-HCl (pH 7.5), at 25°C	Nicotiana langsdorffii
0.1186	-	(S)-nicotine	isoform CYP82E3, in 50 mM Tris-HCl (pH 7.5), at 25°C	Nicotiana alata

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(S)-nicotine + [reduced NADPH-hemoprotein reductase] + O2	Nicotiana alata	-	(S)-nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
(S)-nicotine + [reduced NADPH-hemoprotein reductase] + O2	Nicotiana langsdorffii	-	(S)-nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Nicotiana alata	J7M2E3	-	-
Nicotiana alata	J7M5N9	-	-
Nicotiana alata	J7MB22	-	-
Nicotiana alata	J7MDR0	-	-
Nicotiana langsdorffii	J7M8Q0	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Nicotiana alata	-
leaf	-	Nicotiana langsdorffii	-
root	strong expression	Nicotiana alata	-
root	negligible expression	Nicotiana langsdorffii	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-nicotine + [reduced NADPH-hemoprotein reductase] + O2	-	Nicotiana alata	(S)-nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
(S)-nicotine + [reduced NADPH-hemoprotein reductase] + O2	-	Nicotiana langsdorffii	(S)-nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Synonyms

Synonyms	Comment	Organism
CYP82E	-	Nicotiana alata
CYP82E	-	Nicotiana langsdorffii
CYP82E1	isoform	Nicotiana langsdorffii
CYP82E1	isoform	Nicotiana alata
CYP82E2	isoform	Nicotiana alata
CYP82E3	isoform	Nicotiana alata
CYP82E4	isoform	Nicotiana alata

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome P450	-	Nicotiana alata
cytochrome P450	-	Nicotiana langsdorffii
NADPH	-	Nicotiana alata
NADPH	-	Nicotiana langsdorffii

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Release 2023.1 (January 2023)