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Literature summary for 1.14.14.B11 extracted from

  • Gavilano, L.B.; Siminszky, B.
    Isolation and characterization of the cytochrome P450 gene CYP82E5v2 that mediates nicotine to nornicotine conversion in the green leaves of tobacco (2007), Plant Cell Physiol., 48, 1567-1574.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.056
-
Nicotine pH 7.1, 23°C Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Nicotiana tabacum
-
-

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum A9QNE6 isoform CYP82E5v
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf high expression in green leaf Nicotiana tabacum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nicotine + [reduced NADPH-hemoprotein reductase] + O2
-
Nicotiana tabacum nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
Nicotiana tabacum