Literature summary for 1.14.14.B11 extracted from

Chakrabarti, M.; Meekins, K.M.; Gavilano, L.B.; Siminszky, B.
Inactivation of the cytochrome P450 gene CYP82E2 by degenerative mutations was a key event in the evolution of the alkaloid profile of modern tobacco (2007), New Phytol., 175, 565-574.

Search for more literature for 1.14.14.B11

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Saccharomyces cerevisiae	Nicotiana sylvestris

Protein Variants

Protein Variants	Comment	Organism
E375K	loss of catalytic activity	Nicotiana sylvestris
additional information	analysis of polymorphic variations of the CYP82E2 orthologs in Nicotiana sylvestris and various Nicotiana tabacum genotypes	Nicotiana sylvestris
W422L	loss of catalytic activity	Nicotiana sylvestris

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.015	-	Nicotine	pH 7.1, 23°C	Nicotiana sylvestris

Organism

Organism	UniProt	Comment	Textmining
Nicotiana sylvestris	A7KMF3	isoform CYP82E2	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	highly active in senescing leaf	Nicotiana sylvestris	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
nicotine + [reduced NADPH-hemoprotein reductase] + O2	-	Nicotiana sylvestris	nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

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Release 2023.1 (January 2023)